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  • Provedli jsme podrobný průzkum mitochondriální (mtch) a chloroplastové (cp) DNA diversity v osmi populacích Silene vulgaris ze Střední Evropy s cílem porovnání se dříve publikovanými údaji o této diverzitě v introdukovaném areálu S. vulgaris v USA. Variabilita mtch DNA v okolí genu coxI byla stanovena pomocí Southernovy hybridizace a RFLP. Variabilita cpDNA byla určena sekvenováním intergenové oblasti mezi geny psbA a trnH. Nalezli jsme 30 různých mtch haplotypů a 26 různých chloroplastových haplotypů mezi 86 jedinci. Celková genetická diverzita h (0,941 pro mtch markery a 0,893 pro cp markery) a vnitropopulační diverzita byly byly vyšší než u předcházejících popolačních studií S. vulgaris v Evropě či USA. Zjistili jsme take překvapivě vysolý podíl privátních alel – vice než 90% u obou typů markerů. Většina námi sledovaných populací měla značnou velikost a byla umístěna v realtivně zachovalých lučních porostech. Pomalá rychlost výměny (turnover) evropských populace je zvažována jako... (cs)
  • A detailed survey of mitochondrial and chloroplast diversity in eight populations of Silene vulgaris from Central Europe was conducted for comparison with previously published data on diversity from S. vulgaris populations in the introduced range. Mitochondrial DNA (mtDNA) variation around the coxI gene was assessed with Southern blotting/restriction fragment length polymorphism methods. Chloroplast variation was assessed by sequencing the intergenic spacer separating the trnH and psbA genes. Thirty mtDNA haplotypes and 24 chloroplast DNA (cpDNA) haplotypes were found within 86 individuals. The overall genetic diversity h (0.941 for mitochondrial, and 0.893 for chloroplast markers) and within-population diversity were higher than reported in previous population studies of S. vulgaris in the USA and Europe. The frequency of private alleles was surprisingly high - more than 90% for both kinds of markers. Most of our populations were large and located in relatively undisturbed meadows,...
  • A detailed survey of mitochondrial and chloroplast diversity in eight populations of Silene vulgaris from Central Europe was conducted for comparison with previously published data on diversity from S. vulgaris populations in the introduced range. Mitochondrial DNA (mtDNA) variation around the coxI gene was assessed with Southern blotting/restriction fragment length polymorphism methods. Chloroplast variation was assessed by sequencing the intergenic spacer separating the trnH and psbA genes. Thirty mtDNA haplotypes and 24 chloroplast DNA (cpDNA) haplotypes were found within 86 individuals. The overall genetic diversity h (0.941 for mitochondrial, and 0.893 for chloroplast markers) and within-population diversity were higher than reported in previous population studies of S. vulgaris in the USA and Europe. The frequency of private alleles was surprisingly high - more than 90% for both kinds of markers. Most of our populations were large and located in relatively undisturbed meadows,... (en)
Title
  • Comparison between mitochondrial and chloroplast DNA variation in the native range of Silene vulgaris
  • Porovnání proměnlivosti chloroplastové a mitochondriální DNA v původním areálu Silene vulgaris (cs)
  • Comparison between mitochondrial and chloroplast DNA variation in the native range of Silene vulgaris (en)
skos:prefLabel
  • Comparison between mitochondrial and chloroplast DNA variation in the native range of Silene vulgaris
  • Porovnání proměnlivosti chloroplastové a mitochondriální DNA v původním areálu Silene vulgaris (cs)
  • Comparison between mitochondrial and chloroplast DNA variation in the native range of Silene vulgaris (en)
skos:notation
  • RIV/61389030:_____/04:00109140!RIV/2005/AV0/A56005/N
http://linked.open.../vavai/riv/strany
  • 2909;2919
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(AV0Z5038910)
http://linked.open...iv/cisloPeriodika
  • 10
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 558189
http://linked.open...ai/riv/idVysledku
  • RIV/61389030:_____/04:00109140
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Caryophyllaceae; chloroplast; Europe (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV
  • [3CC87399166C]
http://linked.open...i/riv/nazevZdroje
  • Molecular Ecology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 13
http://linked.open...iv/tvurceVysledku
  • Štorchová, Helena
  • Olson, M.
http://linked.open...n/vavai/riv/zamer
issn
  • 0962-1083
number of pages
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