About: Cytokinin-induced upregulation of cytokinin oxidase activity in tobacco includes changes in enzyme glycosylation and secretion.

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About: Cytokinin-induced upregulation of cytokinin oxidase activity in tobacco includes changes in enzyme glycosylation and secretion. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Regulation of cytokinin oxidase (CKX) activity in relation to enzyme glycosylation and secretion was studied in wild-type (WT) and transgenic conditionally isopentenyltransferase gene (ipt)-expressing (IPT) tobacco (Nicotiana tabacum L. cv. Wisconsin 38) cell suspensions, calli and leaves. An increase in endogenous cytokinin content due to the tetracycline (Tc)-induced derepression of the ipt gene transcription or surface application of N6-benzylaminopurine (BA) resulted in significant enhancement of CKX activity in all these plant materials. As revealed by Concanavalin A-Sepharose 4B chromatography the cytokinin-induced enhancement of CKX activity was associated predominantly with the N-glycoform of the enzyme (10- to 15-fold increase) in calli and leaves. Application of BA to the culture media of WT and IPT cell suspensions and the derepression of the ipt by Tc substantially enhanced endogenous levels of isoprenoid cytokinins and CKX activity in both cells and the culture medium. Most CKX activity i Regulation of cytokinin oxidase (CKX) activity in relation to enzyme glycosylation and secretion was studied in wild-type (WT) and transgenic conditionally isopentenyltransferase gene (ipt)-expressing (IPT) tobacco (Nicotiana tabacum L. cv. Wisconsin 38) cell suspensions, calli and leaves. An increase in endogenous cytokinin content due to the tetracycline (Tc)-induced derepression of the ipt gene transcription or surface application of N6-benzylaminopurine (BA) resulted in significant enhancement of CKX activity in all these plant materials. As revealed by Concanavalin A-Sepharose 4B chromatography the cytokinin-induced enhancement of CKX activity was associated predominantly with the N-glycoform of the enzyme (10- to 15-fold increase) in calli and leaves. Application of BA to the culture media of WT and IPT cell suspensions and the derepression of the ipt by Tc substantially enhanced endogenous levels of isoprenoid cytokinins and CKX activity in both cells and the culture medium. Most CKX activity i (en)
Title	Cytokinin-induced upregulation of cytokinin oxidase activity in tobacco includes changes in enzyme glycosylation and secretion. Cytokinin-induced upregulation of cytokinin oxidase activity in tobacco includes changes in enzyme glycosylation and secretion. (en)
skos:prefLabel	Cytokinin-induced upregulation of cytokinin oxidase activity in tobacco includes changes in enzyme glycosylation and secretion. Cytokinin-induced upregulation of cytokinin oxidase activity in tobacco includes changes in enzyme glycosylation and secretion. (en)
skos:notation	RIV/61389030:_____/03:56033106!RIV/2004/GA0/A56004/N
http://linked.open.../vavai/riv/strany	11;21
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA206/02/0967), P(GA522/00/1346), P(GA522/99/1130), P(IAA6038002), Z(AV0Z5038910)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Motyka, Václav Vaňková, Radomíra Petrášek, Jan Kamínek, Miroslav Čapková, Věra
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	602576
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/03:56033106
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cytokinin oxidase; Nicotiana tabacum; N6-benzylaminopurine (en)
http://linked.open.../riv/klicoveSlovo	N6-benzylaminopurine cytokinin oxidase Nicotiana tabacum
http://linked.open...odStatuVydavatele	DK - Dánské království
http://linked.open...ontrolniKodProRIV	[132322BCB6C9]
http://linked.open...i/riv/nazevZdroje	Physiologia Plantarum
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Targeting of cytokinin oxidase and its role in regulation of plant development Function and physiol.significance of cytokinin oxidase& zeatin reductase in control of active cytokinin levels and their relation to productivity of crop plants The role of glycosylation in regulation of cytokinin levels and control of morphogenic responses of selected crop plants Metabolic inactivation of cytokinins in plants and its physiological significance
http://linked.open...UplatneniVysledku	2003
http://linked.open...v/svazekPeriodika	117
http://linked.open...iv/tvurceVysledku	Motyka, Václav Petrášek, Jan Schmülling, T. Vaňková, Radomíra Kamínek, Miroslav Čapková, Věra
http://linked.open...n/vavai/riv/zamer	Physiological and genetical bases of plant development, cell cycle, morphogenesis, stress reactions and biotechnologies. Genome organisation and function
issn	0031-9317
number of pages	11 (xsd:int)

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