About: The detection of recombinant, tuber necrosing isolates of Potato virus Y (PVYNTN) using a three-primer PCR based in the coat protein gene.

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About: The detection of recombinant, tuber necrosing isolates of Potato virus Y (PVYNTN) using a three-primer PCR based in the coat protein gene. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A simple and reliable procedure for reverse transcription-polymerase chain reaction (RT-PCR) detection and strain differentiation of Potato virus Y (PVY) was developed. Three primers were designed within the coat protein (CP) and nuclear inclusion protein b (NIb) region, exploiting a single base polymorphism identified as being present in all the recombinant PVYNTN isolates published. Samples infected with PVY produce a single band of 569 bp, while isolates belonging to PVYNTN strain give an additional band of 334 bp. The technique was tested on a collection of well-characterised isolates of PVY from a range of strains and was found to detect all of the isolates reported as belonging to the PVYNTN strain. All of the isolates detected possess a recombination event within the coat protein. Further sequence analysis revealed that all the recombinant PVYNTN isolates reported thus far would be detected using this assay, whilst isolates thought to be PVYNTN that do not possess the coat protein recombination A simple and reliable procedure for reverse transcription-polymerase chain reaction (RT-PCR) detection and strain differentiation of Potato virus Y (PVY) was developed. Three primers were designed within the coat protein (CP) and nuclear inclusion protein b (NIb) region, exploiting a single base polymorphism identified as being present in all the recombinant PVYNTN isolates published. Samples infected with PVY produce a single band of 569 bp, while isolates belonging to PVYNTN strain give an additional band of 334 bp. The technique was tested on a collection of well-characterised isolates of PVY from a range of strains and was found to detect all of the isolates reported as belonging to the PVYNTN strain. All of the isolates detected possess a recombination event within the coat protein. Further sequence analysis revealed that all the recombinant PVYNTN isolates reported thus far would be detected using this assay, whilst isolates thought to be PVYNTN that do not possess the coat protein recombination (en)
Title	The detection of recombinant, tuber necrosing isolates of Potato virus Y (PVYNTN) using a three-primer PCR based in the coat protein gene. The detection of recombinant, tuber necrosing isolates of Potato virus Y (PVYNTN) using a three-primer PCR based in the coat protein gene. (en)
skos:prefLabel	The detection of recombinant, tuber necrosing isolates of Potato virus Y (PVYNTN) using a three-primer PCR based in the coat protein gene. The detection of recombinant, tuber necrosing isolates of Potato virus Y (PVYNTN) using a three-primer PCR based in the coat protein gene. (en)
skos:notation	RIV/61389030:_____/03:56033082!RIV/2004/GA0/A56004/N
http://linked.open.../vavai/riv/strany	63;68
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA522/98/1512), P(QC1167), Z(AV0Z5038910)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Čeřovská, Noemi Moravec, Tomáš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	603287
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/03:56033082
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Potato virus Y; Three primer PCR; Coat protein gene (en)
http://linked.open.../riv/klicoveSlovo	Coat protein gene Three primer PCR Potato virus Y
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[D8FC44B63CF5]
http://linked.open...i/riv/nazevZdroje	Journal of Virological Methods
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Identification, differentiation and characterization of viruses evoking necroses of potato tubers. Charakterization of immunochemical variability of various isolates of potato virus y causing potato tuber necrosis ringspot disease (PVY NTN)
http://linked.open...UplatneniVysledku	2003
http://linked.open...v/svazekPeriodika	109
http://linked.open...iv/tvurceVysledku	Moravec, Tomáš Čeřovská, Noemi Boonham, N.
http://linked.open...n/vavai/riv/zamer	Physiological and genetical bases of plant development, cell cycle, morphogenesis, stress reactions and biotechnologies. Genome organisation and function
issn	0166-0934
number of pages	6 (xsd:int)

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