About: 24-EPIBRASSINOLIDE AT SUBNANOMOLAR CONCENTRATIONS MODULATES GROWTH AND PRODUCTION CHARACTERISTICS OF A MOUSE HYBRIDOMA.

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A mouse hybridoma was cultured either in standard serum-free medium, or in medium diluted to 30%, in which the cells underwent nutritional stress. Steady-state parameters of semicontinuous cultures conducted at 24-epibrassinolide concentrations from 10-16 to 10-9 mol l-1 were evaluated. Typical effects of the agent found both in standard and in diluted media were (i) increase in the value of mitochondrial membrane potential, (ii), drop of intracellular antibody level, (iii) increase in the fraction of the cells in the G0/G1 phase, and (iv) decrease in the fraction of the cells in the S phase. Alleviation of nutritional stress manifested itself in cultures conducted in diluted media. At the lowest brassinosteroid concentrations, the number of steroid molecules in the culture was of the same order of magnitude as the number of viable cells in the culture. This implies involvement of a complex cascade mechanism, through which the steroid molecule induces alterations in gene expression leading finally to A mouse hybridoma was cultured either in standard serum-free medium, or in medium diluted to 30%, in which the cells underwent nutritional stress. Steady-state parameters of semicontinuous cultures conducted at 24-epibrassinolide concentrations from 10-16 to 10-9 mol l-1 were evaluated. Typical effects of the agent found both in standard and in diluted media were (i) increase in the value of mitochondrial membrane potential, (ii), drop of intracellular antibody level, (iii) increase in the fraction of the cells in the G0/G1 phase, and (iv) decrease in the fraction of the cells in the S phase. Alleviation of nutritional stress manifested itself in cultures conducted in diluted media. At the lowest brassinosteroid concentrations, the number of steroid molecules in the culture was of the same order of magnitude as the number of viable cells in the culture. This implies involvement of a complex cascade mechanism, through which the steroid molecule induces alterations in gene expression leading finally to (en)
Title	24-EPIBRASSINOLIDE AT SUBNANOMOLAR CONCENTRATIONS MODULATES GROWTH AND PRODUCTION CHARACTERISTICS OF A MOUSE HYBRIDOMA. 24-EPIBRASSINOLIDE AT SUBNANOMOLAR CONCENTRATIONS MODULATES GROWTH AND PRODUCTION CHARACTERISTICS OF A MOUSE HYBRIDOMA. (en)
skos:prefLabel	24-EPIBRASSINOLIDE AT SUBNANOMOLAR CONCENTRATIONS MODULATES GROWTH AND PRODUCTION CHARACTERISTICS OF A MOUSE HYBRIDOMA. 24-EPIBRASSINOLIDE AT SUBNANOMOLAR CONCENTRATIONS MODULATES GROWTH AND PRODUCTION CHARACTERISTICS OF A MOUSE HYBRIDOMA. (en)
skos:notation	RIV/61389030:_____/03:56033053!RIV/2004/GA0/A56004/N
http://linked.open.../vavai/riv/strany	2190;2200
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/01/0083), P(OC 844.10), Z(AV0Z4055905), Z(AV0Z5038910)
http://linked.open...iv/cisloPeriodika	11
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Franěk, František
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	636792
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/03:56033053
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Steroids; Brassinosteroids; 24-Epibrassinolides (en)
http://linked.open.../riv/klicoveSlovo	Steroids 24-Epibrassinolides Brassinosteroids
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[A458ECC81325]
http://linked.open...i/riv/nazevZdroje	Collection of Czechoslovak Chemical Communications
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Structure activity relationship of brassinollide-type plant growth regulators http://linked.opendata.cz/resource/domain/vavai/projekt/OC%20844.10
http://linked.open...UplatneniVysledku	2003
http://linked.open...v/svazekPeriodika	68
http://linked.open...iv/tvurceVysledku	Eckschlager, T. Kohout, Ladislav Franěk, František
http://linked.open...n/vavai/riv/zamer	Physiological and genetical bases of plant development, cell cycle, morphogenesis, stress reactions and biotechnologies. Genome organisation and function Chemical principles of selected biological phenomena in medicine and ecology
issn	0010-0765
number of pages	11 (xsd:int)

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