About: Production of Polyclonal Antibodies to a Recombinant Coat Protein of Potato mop-top virus.     Goto   Sponge   NotDistinct   Permalink

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Description
  • The coat protein (CP) coding regions of two Czech Potato mop-top virus (PMTV) isolates were sequenced and shown to be identical. One, the Korneta isolate CP gene, was cloned in several expression vectors. The recombinant PMTV-CP was expressed in Escherichia coli and the purified recombinant protein was used to produce PMTV-specific polyclonal antibodies. The antiserum had a titre of 1 : 2000 in an indirect enzyme-linked immunosorbent assay (ELISA) and reacted specifically in immunoblotting and IPTA- ELISA (indirect plate-trapped antigen (PTA)-ELISA).
  • The coat protein (CP) coding regions of two Czech Potato mop-top virus (PMTV) isolates were sequenced and shown to be identical. One, the Korneta isolate CP gene, was cloned in several expression vectors. The recombinant PMTV-CP was expressed in Escherichia coli and the purified recombinant protein was used to produce PMTV-specific polyclonal antibodies. The antiserum had a titre of 1 : 2000 in an indirect enzyme-linked immunosorbent assay (ELISA) and reacted specifically in immunoblotting and IPTA- ELISA (indirect plate-trapped antigen (PTA)-ELISA). (en)
Title
  • Production of Polyclonal Antibodies to a Recombinant Coat Protein of Potato mop-top virus.
  • Production of Polyclonal Antibodies to a Recombinant Coat Protein of Potato mop-top virus. (en)
skos:prefLabel
  • Production of Polyclonal Antibodies to a Recombinant Coat Protein of Potato mop-top virus.
  • Production of Polyclonal Antibodies to a Recombinant Coat Protein of Potato mop-top virus. (en)
skos:notation
  • RIV/61389030:_____/03:56033041!RIV/2004/GA0/A56004/N
http://linked.open.../vavai/riv/strany
  • 195;200
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA522/01/1121), Z(AV0Z5038910)
http://linked.open...iv/cisloPeriodika
  • 4
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 623570
http://linked.open...ai/riv/idVysledku
  • RIV/61389030:_____/03:56033041
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • potato mop-top virus; recombinant coat protein; Escherichia Coli (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV
  • [B2587E036A1F]
http://linked.open...i/riv/nazevZdroje
  • Journal of phytopathology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...ocetUcastnikuAkce
http://linked.open...nichUcastnikuAkce
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 151
http://linked.open...iv/tvurceVysledku
  • Moravec, Tomáš
  • Dědič, P.
  • Čeřovská, Noemi
  • Filigarová, Marie
  • Rosecká, Pavla
http://linked.open...n/vavai/riv/zamer
issn
  • 0931-1785
number of pages
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