About: Development of molecular cytogenetics and physical mapping of ribosomal RNA genes in Lupinus.

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About: Development of molecular cytogenetics and physical mapping of ribosomal RNA genes in Lupinus. Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Identification of individual chromosomes in Lupinus is not possible due to gradient in size and in similar morphology. To overcome this problem, molecular cytogenetics was developed for Lupinus. As an initial step in karyotype analysis, fluorescent in situ hybridization (FISH) was performed to determine genomic distribution of rRNA genes in L. hispanicus, L. luteus and L. x hispanicoluteus.It was found that all three diploid species posses two chromosome pairs carrying 18S-5.8S-25S rDNA and one chromosome pair carrying 5S rDNA. The use of probes for DNA permitted unambiguous identification of three different pairs of chromosomes and revealed conservation of the number of rDNA loci among the three species. The study represents the first step in physical mapping of Lupinus genome through FISH by providing distinct chromosome lendmarks. Identification of individual chromosomes in Lupinus is not possible due to gradient in size and in similar morphology. To overcome this problem, molecular cytogenetics was developed for Lupinus. As an initial step in karyotype analysis, fluorescent in situ hybridization (FISH) was performed to determine genomic distribution of rRNA genes in L. hispanicus, L. luteus and L. x hispanicoluteus.It was found that all three diploid species posses two chromosome pairs carrying 18S-5.8S-25S rDNA and one chromosome pair carrying 5S rDNA. The use of probes for DNA permitted unambiguous identification of three different pairs of chromosomes and revealed conservation of the number of rDNA loci among the three species. The study represents the first step in physical mapping of Lupinus genome through FISH by providing distinct chromosome lendmarks. (en)
Title	Development of molecular cytogenetics and physical mapping of ribosomal RNA genes in Lupinus. Development of molecular cytogenetics and physical mapping of ribosomal RNA genes in Lupinus. (en)
skos:prefLabel	Development of molecular cytogenetics and physical mapping of ribosomal RNA genes in Lupinus. Development of molecular cytogenetics and physical mapping of ribosomal RNA genes in Lupinus. (en)
skos:notation	RIV/61389030:_____/03:56033010!RIV/2004/GA0/A56004/N
http://linked.open.../vavai/riv/strany	211;215
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA521/03/0595), Z(AV0Z5038910)
http://linked.open...iv/cisloPeriodika	N/A
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Doležel, Jaroslav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	603491
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/03:56033010
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	fluorescent in situ hybridization; Lupinus sp.; rRNA genes (en)
http://linked.open.../riv/klicoveSlovo	Lupinus sp. fluorescent in situ hybridization rRNA genes
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[0F800FD646B9]
http://linked.open...i/riv/nazevZdroje	Biologia Plantarum
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Targeted isolation of molecular markers and comparative genome mapping in chickpea, field bean and garden pea
http://linked.open...UplatneniVysledku	2003
http://linked.open...v/svazekPeriodika	46
http://linked.open...iv/tvurceVysledku	Doležel, Jaroslav Naganowska, B. Swiecicki, W. K.
http://linked.open...n/vavai/riv/zamer	Physiological and genetical bases of plant development, cell cycle, morphogenesis, stress reactions and biotechnologies. Genome organisation and function
issn	0006-3134
number of pages	5 (xsd:int)

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