Attributes | Values |
---|
rdf:type
| |
Description
| - Changes in levels of endogenous cytokinins and activity of cytokinin oxidase (EC 1.4.3.6) were followed during the cell cycle progression in large-scale (ca. 30 g FW) synchronized culture of the tobacco cell line BY-2. Synchronization was induced either by 4 mM hydroxyurea or 15 iM aphidicolin alone (a single block of the cell cycle at G,/S) or in combination with 1 uM oryzalin (double blocking at G,/S and Gz/M). Up to 46% synchrony was obtained. Two distinct cytokinin peaks were detected at 0-2 h and 8.5-9 h after the release from the first block coinciding with the beginning of the S phase and with the onset of mitosis, respectively. Interestingly, as identified by MS/MS, very sharp and transient premitotic peak of cytokinins contained, in addition to fra/u-zeatin, also considerable amounts of cu-zeatin and zeatin-0-glucoside. Cytokinin oxidase activity exhibited conspicuous maximum parallel to the first peak of cytokinin accumulation and deep minimum corresponding to the culmination of mitotic inde
- Changes in levels of endogenous cytokinins and activity of cytokinin oxidase (EC 1.4.3.6) were followed during the cell cycle progression in large-scale (ca. 30 g FW) synchronized culture of the tobacco cell line BY-2. Synchronization was induced either by 4 mM hydroxyurea or 15 iM aphidicolin alone (a single block of the cell cycle at G,/S) or in combination with 1 uM oryzalin (double blocking at G,/S and Gz/M). Up to 46% synchrony was obtained. Two distinct cytokinin peaks were detected at 0-2 h and 8.5-9 h after the release from the first block coinciding with the beginning of the S phase and with the onset of mitosis, respectively. Interestingly, as identified by MS/MS, very sharp and transient premitotic peak of cytokinins contained, in addition to fra/u-zeatin, also considerable amounts of cu-zeatin and zeatin-0-glucoside. Cytokinin oxidase activity exhibited conspicuous maximum parallel to the first peak of cytokinin accumulation and deep minimum corresponding to the culmination of mitotic inde (en)
|
Title
| - Transient accumulation of cis- and trans-zeatin type cytokinins and its relation to cytokinin oxidase activity during cell cycle of synchronized tobacco BY-2 cells.
- Transient accumulation of cis- and trans-zeatin type cytokinins and its relation to cytokinin oxidase activity during cell cycle of synchronized tobacco BY-2 cells. (en)
|
skos:prefLabel
| - Transient accumulation of cis- and trans-zeatin type cytokinins and its relation to cytokinin oxidase activity during cell cycle of synchronized tobacco BY-2 cells.
- Transient accumulation of cis- and trans-zeatin type cytokinins and its relation to cytokinin oxidase activity during cell cycle of synchronized tobacco BY-2 cells. (en)
|
skos:notation
| - RIV/61389030:_____/02:56023019!RIV/2003/GA0/A56003/N
|
http://linked.open.../vavai/riv/strany
| |
http://linked.open...avai/riv/aktivita
| |
http://linked.open...avai/riv/aktivity
| - P(GA522/99/1130), P(GV206/96/K188), P(IAA6038002), Z(AV0Z5038910)
|
http://linked.open...iv/cisloPeriodika
| |
http://linked.open...vai/riv/dodaniDat
| |
http://linked.open...aciTvurceVysledku
| |
http://linked.open.../riv/druhVysledku
| |
http://linked.open...iv/duvernostUdaju
| |
http://linked.open...titaPredkladatele
| |
http://linked.open...dnocenehoVysledku
| |
http://linked.open...ai/riv/idVysledku
| - RIV/61389030:_____/02:56023019
|
http://linked.open...riv/jazykVysledku
| |
http://linked.open.../riv/klicovaSlova
| - Cis zeatin; Cytokinins; Cytokinin oxidase (en)
|
http://linked.open.../riv/klicoveSlovo
| |
http://linked.open...odStatuVydavatele
| |
http://linked.open...ontrolniKodProRIV
| |
http://linked.open...i/riv/nazevZdroje
| - Plant Physiology and Biochemistry
|
http://linked.open...in/vavai/riv/obor
| |
http://linked.open...ichTvurcuVysledku
| |
http://linked.open...cetTvurcuVysledku
| |
http://linked.open...ocetUcastnikuAkce
| |
http://linked.open...nichUcastnikuAkce
| |
http://linked.open...vavai/riv/projekt
| |
http://linked.open...UplatneniVysledku
| |
http://linked.open...v/svazekPeriodika
| |
http://linked.open...iv/tvurceVysledku
| - Dobrev, Petre
- Malbeck, Jiří
- Motyka, Václav
- Trávníčková, Alena
- Vaňková, Radomíra
- Gaudinová, Alena
- Kamínek, Miroslav
|
http://linked.open...n/vavai/riv/zamer
| |
issn
| |
number of pages
| |