About: Sorting of mitotic chromosomes in common wheat (Triticum aestivum L.) using flow cytometry

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Sorting of mitotic chromosomes in common wheat (Triticum aestivum L.) using flow cytometry. (cs) The aim of this study was to develop an improved procedure for preparation of chromosome suspensions, and to evaluate the potential of flow cytometry for chromosome sorting in wheat. Suspensions of intact chromosomes were prepared by mechanical homogenization of synchronized root tips after mild fixation with formaldehyde. Histograms of relative fluorescence intensity (flow karyotypes) obtained after the analysis of DAPI-stained chromosomes were characterized and the chromosome content of all peaks on wheat flow karyotype was determined for the first time. Only chromosome 3B could be discriminated on flow karyotypes of wheat lines with standard karyotype. Remaining chromosomes formed three composite peaks and could be sorted only as groups. Chromosome 3B could be sorted at purity >95% as determined by microscopic evaluation of sorted fractions that were labeled using C-PRINS with primers for GAA microsatellites and for Afa repeats, respectively. The aim of this study was to develop an improved procedure for preparation of chromosome suspensions, and to evaluate the potential of flow cytometry for chromosome sorting in wheat. Suspensions of intact chromosomes were prepared by mechanical homogenization of synchronized root tips after mild fixation with formaldehyde. Histograms of relative fluorescence intensity (flow karyotypes) obtained after the analysis of DAPI-stained chromosomes were characterized and the chromosome content of all peaks on wheat flow karyotype was determined for the first time. Only chromosome 3B could be discriminated on flow karyotypes of wheat lines with standard karyotype. Remaining chromosomes formed three composite peaks and could be sorted only as groups. Chromosome 3B could be sorted at purity >95% as determined by microscopic evaluation of sorted fractions that were labeled using C-PRINS with primers for GAA microsatellites and for Afa repeats, respectively. (en)
Title	Sorting of mitotic chromosomes in common wheat (Triticum aestivum L.) using flow cytometry Sorting of mitotic chromosomes in common wheat (Triticum aestivum L.) using flow cytometry (en) Sorting of mitotic chromosomes in common wheat (Triticum aestivum L.) using flow cytometry (cs)
skos:prefLabel	Sorting of mitotic chromosomes in common wheat (Triticum aestivum L.) using flow cytometry Sorting of mitotic chromosomes in common wheat (Triticum aestivum L.) using flow cytometry (en) Sorting of mitotic chromosomes in common wheat (Triticum aestivum L.) using flow cytometry (cs)
skos:notation	RIV/61389030:_____/01:00100780!RIV/2005/AV0/A56005/N
http://linked.open.../vavai/riv/strany	531;536
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(IBS5038104), Z(AV0Z5038910)
http://linked.open...vai/riv/dodaniDat	2005
http://linked.open...aciTvurceVysledku	Lysák, Martin Doležel, Jaroslav Šimková, Hana Kubaláková, Marie Číhalíková, Jarmila Vrána, Jan
http://linked.open.../riv/druhVysledku	C - Kapitola v knize
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	696429
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/01:00100780
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	chromosome isolation; chromosome sorting; flow cytometry (en)
http://linked.open.../riv/klicoveSlovo	chromosome isolation flow cytometry chromosome sorting
http://linked.open...ontrolniKodProRIV	[2626CB0DD968]
http://linked.open...i/riv/mistoVydani	Dordrecht
http://linked.open...i/riv/nazevZdroje	Wheat in a Global Environment
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Molecular cytogenetic and cytometric methods for breeding of grasses and trifolium
http://linked.open...UplatneniVysledku	2001
http://linked.open...iv/tvurceVysledku	Doležel, Jaroslav Kubaláková, Marie Lysák, Martin Číhalíková, Jarmila Šimková, Hana Vrána, Jan Bedö, Z. , Láng, L.
http://linked.open...n/vavai/riv/zamer	Physiological and genetical bases of plant development, cell cycle, morphogenesis, stress reactions and biotechnologies. Genome organisation and function
number of pages	6 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Kluwer

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