About: Alternative polymer precipitants for protein crystallization

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A set of 16 inexpensive and commercially available polymer precipitants were tested for protein crystallization. Eight of them were found suitable: polyethylene glycol dimethyl ether of molecular weight (MW) 500, 1000 and 2000; di[poly(ethylene glycol)] adipate, MW 900; poly(ethylene glycol-ran-propylene glycol), MW 2500 and 12000; poly(acrylic acid) sodium salt, MW 2100; and polyethylene glycol methyl ether methacrylate, MW 1100. Two new crystallization screens, PolyA and PolyB, were formulated using these eight polymers, each containing 96 solutions – four polymers in combination with 24 common salts and buffers, covering pH values from 4.5 to 9.0. The screens were tested on 29 proteins, 21 of which were crystallized. The tests confirmed the applicability of the eight polymers as precipitants for protein crystallization. A set of 16 inexpensive and commercially available polymer precipitants were tested for protein crystallization. Eight of them were found suitable: polyethylene glycol dimethyl ether of molecular weight (MW) 500, 1000 and 2000; di[poly(ethylene glycol)] adipate, MW 900; poly(ethylene glycol-ran-propylene glycol), MW 2500 and 12000; poly(acrylic acid) sodium salt, MW 2100; and polyethylene glycol methyl ether methacrylate, MW 1100. Two new crystallization screens, PolyA and PolyB, were formulated using these eight polymers, each containing 96 solutions – four polymers in combination with 24 common salts and buffers, covering pH values from 4.5 to 9.0. The screens were tested on 29 proteins, 21 of which were crystallized. The tests confirmed the applicability of the eight polymers as precipitants for protein crystallization. (en)
Title	Alternative polymer precipitants for protein crystallization Alternative polymer precipitants for protein crystallization (en)
skos:prefLabel	Alternative polymer precipitants for protein crystallization Alternative polymer precipitants for protein crystallization (en)
skos:notation	RIV/61389013:_____/10:00345206!RIV11-GA0-61389013
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA305/07/1073), P(IAA500500701), Z(AV0Z40500505)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Štěpánková, Andrea Dohnálek, Jan Hašek, Jindřich Skálová, Tereza Dušková, Jarmila Kolenko, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav makromolekulární chemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	246039
http://linked.open...ai/riv/idVysledku	RIV/61389013:_____/10:00345206
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	polymer precipitants; protein crystallization; X-ray crystallography (en)
http://linked.open.../riv/klicoveSlovo	X-ray crystallography protein crystallization polymer precipitants
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[3B161B85AB3B]
http://linked.open...i/riv/nazevZdroje	Journal of Applied Crystallography
http://linked.open...in/vavai/riv/obor	CD
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Molecular interactions of polymers for biomedical applications Self-organization, crystallization and structure determination of protein based macromolecular systems
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	43
http://linked.open...iv/tvurceVysledku	Dohnálek, Jan Dušková, Jarmila Hašek, Jindřich Skálová, Tereza Štěpánková, Andrea Kolenko, Petr
http://linked.open...ain/vavai/riv/wos	000279429500011
http://linked.open...n/vavai/riv/zamer	Progresivní makromolekulární materiály a supramolekulární systémy: syntéza a studium vlastností, jevů a možností využití pro speciální aplikace a moderní technologie
issn	0021-8898
number of pages	6 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Alternative polymer precipitants for protein crystallization Alternative polymer precipitants for protein crystallization Alternative polymer precipitants for protein crystallization

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