About: Direct astatination of a tumour-binding protein, human epidermal growth factor, using nido-carborane as a prosthetic group.

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About: Direct astatination of a tumour-binding protein, human epidermal growth factor, using nido-carborane as a prosthetic group. Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We have investigated a method for direct astatine labeling of proteins. Binding sites for astatine were created by coupling of a nido-carborane derivative to a protein, the human epidermal growth factor (hEGF), using two different conjugation methods - by glutaraldehyde cross-linking or by introduction of sulfohydryl groups by Traut's reagent with subsequent linking of ANC-1 with m-maleimidobenzoyl-N-hydroxysulfosuccinimide ester. The conjugates were astatinated using the Chloramine-T method in high yield. The best labeling was obtained by the glutaraldehyde conjugate with an average yield of 68+/-9%. In vitro stability tests indicated that the glutaraldehyde conjugated label was as stable as hEGF labeled with astatobenzoate. We have investigated a method for direct astatine labeling of proteins. Binding sites for astatine were created by coupling of a nido-carborane derivative to a protein, the human epidermal growth factor (hEGF), using two different conjugation methods - by glutaraldehyde cross-linking or by introduction of sulfohydryl groups by Traut's reagent with subsequent linking of ANC-1 with m-maleimidobenzoyl-N-hydroxysulfosuccinimide ester. The conjugates were astatinated using the Chloramine-T method in high yield. The best labeling was obtained by the glutaraldehyde conjugate with an average yield of 68+/-9%. In vitro stability tests indicated that the glutaraldehyde conjugated label was as stable as hEGF labeled with astatobenzoate. (en)
Title	Direct astatination of a tumour-binding protein, human epidermal growth factor, using nido-carborane as a prosthetic group. Direct astatination of a tumour-binding protein, human epidermal growth factor, using nido-carborane as a prosthetic group. (en)
skos:prefLabel	Direct astatination of a tumour-binding protein, human epidermal growth factor, using nido-carborane as a prosthetic group. Direct astatination of a tumour-binding protein, human epidermal growth factor, using nido-carborane as a prosthetic group. (en)
skos:notation	RIV/61389005:_____/03:49033071!RIV/2004/AV0/A49004/N
http://linked.open.../vavai/riv/strany	191;197
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(KSK4055109)
http://linked.open...iv/cisloPeriodika	7
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Lebeda, Ondřej
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav jaderné fyziky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	603999
http://linked.open...ai/riv/idVysledku	RIV/61389005:_____/03:49033071
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	neutron-capture therapy; astatine-211 (en)
http://linked.open.../riv/klicoveSlovo	neutron-capture therapy astatine-211
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[C96A41941603]
http://linked.open...i/riv/nazevZdroje	JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
http://linked.open...in/vavai/riv/obor	CH
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Physics, chemistry and informatics for biological, ecological and medicinal applications
http://linked.open...UplatneniVysledku	2003
http://linked.open...v/svazekPeriodika	256
http://linked.open...iv/tvurceVysledku	Lebeda, Ondřej Koziorowski, J. Carlsson, J. Lundqvist, H. Tolmachev, V. Sjostrom, A.
issn	0236-5731
number of pages	7 (xsd:int)

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