About: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry as a tool for fast identification of protein binders in color layers of paintings

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About: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry as a tool for fast identification of protein binders in color layers of paintings Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Identification of materials in color layers of paintings is necessary for correct decisions concerning restoration procedures as well as proving the authenticity of the painting. The proteins are usually important components of the painting layers. In this paper it has been demonstrated that matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) can be used for fast and reliable identification of proteins in color layers even in old, highly aged matrices. The digestion can be easily performed directly on silica wafers which are routinely used for infrared analysis. The amount of material necessary for such an analysis is extremely small. Peptide mass mapping using digestion with trypsin followed by MALDI-TOFMS and identification of the protein was successfully used for determination of the binder from a painting of the 19th century. Identification of materials in color layers of paintings is necessary for correct decisions concerning restoration procedures as well as proving the authenticity of the painting. The proteins are usually important components of the painting layers. In this paper it has been demonstrated that matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) can be used for fast and reliable identification of proteins in color layers even in old, highly aged matrices. The digestion can be easily performed directly on silica wafers which are routinely used for infrared analysis. The amount of material necessary for such an analysis is extremely small. Peptide mass mapping using digestion with trypsin followed by MALDI-TOFMS and identification of the protein was successfully used for determination of the binder from a painting of the 19th century. (en) Magnetit byl připraven mletím mikročástic hematitu a železa a pak studován chemickou analýzou, elektronovou mikroskopií, rtg difrakcí a Mossbauerovou spektroskopií. Magnetit se vyznačoval velkou magnetickou tvrdostí a po dlouhém mletí obsahoval příměs wustitu (FeO). (cs)
Title	Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry as a tool for fast identification of protein binders in color layers of paintings Příprava nanokrystalického magnetitu vysokoenergetickým mletím železa a hematitu (cs) Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry as a tool for fast identification of protein binders in color layers of paintings (en)
skos:prefLabel	Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry as a tool for fast identification of protein binders in color layers of paintings Příprava nanokrystalického magnetitu vysokoenergetickým mletím železa a hematitu (cs) Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry as a tool for fast identification of protein binders in color layers of paintings (en)
skos:notation	RIV/61388980:_____/04:00106530!RIV/2005/AV0/A50005/N
http://linked.open.../vavai/riv/strany	1896;1900
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/04/2091), Z(AV0Z4032918), Z(MSM 223300006)
http://linked.open...iv/cisloPeriodika	17
http://linked.open...vai/riv/dodaniDat	2005
http://linked.open...aciTvurceVysledku	Kučková, Štěpánka
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav anorganické chemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	572544
http://linked.open...ai/riv/idVysledku	RIV/61388980:_____/04:00106530
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	protein binders; mass spectrometry (en)
http://linked.open.../riv/klicoveSlovo	mass spectrometry protein binders
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[673A8BAC2CB1]
http://linked.open...i/riv/nazevZdroje	Rapid Communications in Mass Spectrometry
http://linked.open...in/vavai/riv/obor	CA
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Material and micro-structural features in the dating and technological evaluation of the Mid-European painting
http://linked.open...UplatneniVysledku	2004
http://linked.open...v/svazekPeriodika	18
http://linked.open...iv/tvurceVysledku	Hradilová, J. Hynek, R. Kodíček, M. Kučková, Štěpánka
http://linked.open...n/vavai/riv/zamer	Synthesis and structure of novel (inorganic) compounds, clusters and composites; mechanisms of conversion and transfer in systems involving inorganic substance http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20223300006
issn	0951-4198
number of pages	5 (xsd:int)

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