About: Interspecific variability of class II hydrophobin GEO1 in the genus Geosmithia10     Goto   Sponge   NotDistinct   Permalink

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  • Previous work had shown that a sequence homologous to the gene encoding class II hydrophobin cerato-ulmin from the fungus Ophiostoma novo-ulmi, the causal agent of Dutch Elm Disease (DED), was present in a strain of the unrelated species Geosmithia species 5 (Ascomycota: Hypocreales) isolated from Ulmus minor affected by DED. As both fungi occupy the same habitat, even if different ecological niches, the occurrence of horizontal gene transfer was proposed. In the present work we have analysed for the presence of the cerato-ulmin gene 70 Geosmithia strains representing 29 species, isolated from different host plants and geographic locations. The gene was found in 52.1 % of the strains derived from elm trees, while none of those isolated from nonelms possessed it. The expression of the gene in Geosrnithia was also assessed by real time PCR in different growth conditions (liquid culture, solid culture, elm sawdust, dual culture with O. novo-ulmi), and was found to be extremely low in all conditions tested. On the basis of these results we propose that the cerato-ulmin gene is not functional in Geosmithia, but can be considered instead a marker of more extensive transfers of genetic material as shown in other fungi
  • Previous work had shown that a sequence homologous to the gene encoding class II hydrophobin cerato-ulmin from the fungus Ophiostoma novo-ulmi, the causal agent of Dutch Elm Disease (DED), was present in a strain of the unrelated species Geosmithia species 5 (Ascomycota: Hypocreales) isolated from Ulmus minor affected by DED. As both fungi occupy the same habitat, even if different ecological niches, the occurrence of horizontal gene transfer was proposed. In the present work we have analysed for the presence of the cerato-ulmin gene 70 Geosmithia strains representing 29 species, isolated from different host plants and geographic locations. The gene was found in 52.1 % of the strains derived from elm trees, while none of those isolated from nonelms possessed it. The expression of the gene in Geosrnithia was also assessed by real time PCR in different growth conditions (liquid culture, solid culture, elm sawdust, dual culture with O. novo-ulmi), and was found to be extremely low in all conditions tested. On the basis of these results we propose that the cerato-ulmin gene is not functional in Geosmithia, but can be considered instead a marker of more extensive transfers of genetic material as shown in other fungi (en)
Title
  • Interspecific variability of class II hydrophobin GEO1 in the genus Geosmithia10
  • Interspecific variability of class II hydrophobin GEO1 in the genus Geosmithia10 (en)
skos:prefLabel
  • Interspecific variability of class II hydrophobin GEO1 in the genus Geosmithia10
  • Interspecific variability of class II hydrophobin GEO1 in the genus Geosmithia10 (en)
skos:notation
  • RIV/61388971:_____/14:00436015!RIV15-GA0-61388971
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • I, P(GAP506/11/2302)
http://linked.open...iv/cisloPeriodika
  • 11
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 22548
http://linked.open...ai/riv/idVysledku
  • RIV/61388971:_____/14:00436015
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Ascomycetes; Entomochoric fungi; Gene expression (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV
  • [EFBC5ABFDC67]
http://linked.open...i/riv/nazevZdroje
  • Fungal Biology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 118
http://linked.open...iv/tvurceVysledku
  • Kolařík, Miroslav
  • Bettini, P. P.
  • Comparini, C.
  • Frascella, A.
  • Lutti, S.
  • Pazzagli, L.
  • Scala, A.
  • Scala, F.
http://linked.open...ain/vavai/riv/wos
  • 000345482500002
issn
  • 1878-6146
number of pages
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