About: Novel Aeruginosin-865 from Nostoc sp. as a Potent Anti-inflammatory Agent

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Aeruginosin-865 (Aer-865), isolated from terrestrial cyanobacterium Nostoc sp. Lukesova 30/93, is the first aeruginosin-type peptide containing both a fatty acid and a carbohydrate moiety, and is the first aeruginosin to be found in the genus Nostoc. Mass spectrometry, chemical and spectroscopic analysis as well as one- and two-dimensional NMR and chiral HPLC analysis of Marfey derivatives were applied to determine the peptidic sequence: D-Hpla, D-Leu, 5-OH-Choi, Agma, with hexanoic and mannopyranosyl uronic acid moieties linked to Choi. We used an AlphaLISA assay to measure the levels of proinflammatory mediators IL-8 and ICAM-1 in hTNF-alpha-stimulated HLMVECs. Aer-865 showed significant reduction of both: with EC50 values of (3.51.5) mugmL(-1) ((4.01.7) muM) and (50.013.4) mugmL(-1) ((57.815.5) muM), respectively. Confocal laser scanning microscopy revealed that the anti-inflammatory effect of Aer-865 was directly associated with inhibition of NF-kappaB translocation to the nucleus. Moreover, Aer-865 did not show any cytotoxic effect Aeruginosin-865 (Aer-865), isolated from terrestrial cyanobacterium Nostoc sp. Lukesova 30/93, is the first aeruginosin-type peptide containing both a fatty acid and a carbohydrate moiety, and is the first aeruginosin to be found in the genus Nostoc. Mass spectrometry, chemical and spectroscopic analysis as well as one- and two-dimensional NMR and chiral HPLC analysis of Marfey derivatives were applied to determine the peptidic sequence: D-Hpla, D-Leu, 5-OH-Choi, Agma, with hexanoic and mannopyranosyl uronic acid moieties linked to Choi. We used an AlphaLISA assay to measure the levels of proinflammatory mediators IL-8 and ICAM-1 in hTNF-alpha-stimulated HLMVECs. Aer-865 showed significant reduction of both: with EC50 values of (3.51.5) mugmL(-1) ((4.01.7) muM) and (50.013.4) mugmL(-1) ((57.815.5) muM), respectively. Confocal laser scanning microscopy revealed that the anti-inflammatory effect of Aer-865 was directly associated with inhibition of NF-kappaB translocation to the nucleus. Moreover, Aer-865 did not show any cytotoxic effect (en)
Title	Novel Aeruginosin-865 from Nostoc sp. as a Potent Anti-inflammatory Agent Novel Aeruginosin-865 from Nostoc sp. as a Potent Anti-inflammatory Agent (en)
skos:prefLabel	Novel Aeruginosin-865 from Nostoc sp. as a Potent Anti-inflammatory Agent Novel Aeruginosin-865 from Nostoc sp. as a Potent Anti-inflammatory Agent (en)
skos:notation	RIV/61388971:_____/13:00424616!RIV14-MSM-61388971
http://linked.open...avai/predkladatel	Mikrobiologický ústav AV ČR, v.v.i.
http://linked.open...avai/riv/aktivita	I P V
http://linked.open...avai/riv/aktivity	I, P(ED2.1.00/03.0110), P(LH11129), V
http://linked.open...iv/cisloPeriodika	17
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Bártová, Simona Kuzma, Marek Hrouzek, Pavel Novák, Petr Kopecký, Jiří Kapuscik, Alexandra
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mikrobiologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	92337
http://linked.open...ai/riv/idVysledku	RIV/61388971:_____/13:00424616
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	NMR spectroscopy; anti-inflammatory; cyanobacteria (en)
http://linked.open.../riv/klicoveSlovo	anti-inflammatory NMR spectroscopy cyanobacteria
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[390C58BE106B]
http://linked.open...i/riv/nazevZdroje	Chembiochem
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	10 (xsd:int)
http://linked.open...vavai/riv/projekt	Center of Algal Biotechnology Třeboň (Algatech) Bioactivity of the cyanobacterial secondary metabolites.
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	14
http://linked.open...iv/tvurceVysledku	Hrouzek, Pavel Kopecký, Jiří Kuzma, Marek Novák, Petr Jokela, J. Bártová, Simona Hundsberger, H. Eger, A. Kapuscik, Alexandra Pfluger, M.
http://linked.open...ain/vavai/riv/wos	000330181300016
issn	1439-4227
number of pages	9 (xsd:int)
http://bibframe.org/vocab/doi	10.1002/cbic.201300246

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