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Description
| - LC with atmospheric pressure chemical ionization (ACPI) MS with RP and chiral phase was used for separation of triacylglycerols (TAGs) from yeasts of the genera Candida, Kluyveromyces, Rhodotorula, Saccharomyces, Torulospora, Trichosporon, and Yarrowia. Chiral LC–APCI-MS is based on using two columns in series packed with a 3,5-dimethylphenyl carbamatemodified _-cyclodextrin chiral phase. All regioisomers and enantiomers of TAGs containing one to five double bonds were separated.Molecular species of TAGs, i.e. regioisomers and enantiomers, were identified and quantified by MS/MS. Among the 94 identified TAGs, the most abundant were triolein, oleopalmitoleoolein, and dipalmitoleoolein. In strains producing palmitoleic acid in amounts >25% of total fatty acids (FAs), this acid, or unsaturated FA is bound in sn-1. In strains containing palmitoleic acid at 10–25% total FAs this acid is mainly bound in sn-3, saturated FA being bound in sn-1. Strains containing <10% palmitoleic acid form preferentially symmetrical TAGs.
- LC with atmospheric pressure chemical ionization (ACPI) MS with RP and chiral phase was used for separation of triacylglycerols (TAGs) from yeasts of the genera Candida, Kluyveromyces, Rhodotorula, Saccharomyces, Torulospora, Trichosporon, and Yarrowia. Chiral LC–APCI-MS is based on using two columns in series packed with a 3,5-dimethylphenyl carbamatemodified _-cyclodextrin chiral phase. All regioisomers and enantiomers of TAGs containing one to five double bonds were separated.Molecular species of TAGs, i.e. regioisomers and enantiomers, were identified and quantified by MS/MS. Among the 94 identified TAGs, the most abundant were triolein, oleopalmitoleoolein, and dipalmitoleoolein. In strains producing palmitoleic acid in amounts >25% of total fatty acids (FAs), this acid, or unsaturated FA is bound in sn-1. In strains containing palmitoleic acid at 10–25% total FAs this acid is mainly bound in sn-3, saturated FA being bound in sn-1. Strains containing <10% palmitoleic acid form preferentially symmetrical TAGs. (en)
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Title
| - Identification of regioisomers and enantiomers of triacylglycerols in different yeasts using reversed- and chiral-phase LC–MS
- Identification of regioisomers and enantiomers of triacylglycerols in different yeasts using reversed- and chiral-phase LC–MS (en)
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skos:prefLabel
| - Identification of regioisomers and enantiomers of triacylglycerols in different yeasts using reversed- and chiral-phase LC–MS
- Identification of regioisomers and enantiomers of triacylglycerols in different yeasts using reversed- and chiral-phase LC–MS (en)
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skos:notation
| - RIV/61388971:_____/13:00397491!RIV14-AV0-61388971
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http://linked.open...avai/predkladatel
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/61388971:_____/13:00397491
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - Atmospheric pressure chemical ionization mass spectrometry; Chiral LC; Reversed phase LC (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Journal of Separation Science
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Cajthaml, Tomáš
- Sigler, Karel
- Řezanka, Tomáš
- Čejková, A.
- Kolouchová, I.
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http://linked.open...ain/vavai/riv/wos
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issn
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number of pages
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http://bibframe.org/vocab/doi
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