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  • The freshwater microalga Chlorella vulgaris was grown heterotrophically in fed-batch 50-600-L fermenters at 36A degrees C, on aerated and mixed nutrient solution with urea as a nitrogen and glucose as a carbon and energy source. Cell density increased from the initial value 6.25 to 117.18 g DW L-1 in 32 h in the fermenter 50 L at a mean growth rate 3.52 g DW L-1 h(-1). The DW increase in the fermenter 200 L was from 7.25 to 94.82 g DW L-1 in 26.5 h at a mean growth rate 3.37 g DW L-1 h(-1). Mean specific growth rate mu was about 0.1 h(-1) in the both fermenters, if nutrients and oxygen were adequately supplied. The DW increase in the fermenter 600 L was from 0.8 to 81.6 g DW L-1 in 66.5 h at a mean growth rate 1.22 g DW L-1 h(-1) and mu = 0.07 h(-1). A limitation of the cell growth rate in 600 L fermenter caused by a low dissolved oxygen concentration above cell densities higher than 10 g DW L-1) occurred. Specific growth rate decreased approximately linearly with increasing glucose concentration (25-80 g glucose L-1) at the beginning of cultivation and decreased with the time of cultivation. The cell yield was 0.55-0.69 g DW (g glucose)(-1). The content of proteins, beta-carotene, and chlorophylls in the cells steadily increased and starch content decreased, by keeping aerated and mixed culture another 12 h in fermenter after the cell growth was stopped due to glucose deficiency
  • The freshwater microalga Chlorella vulgaris was grown heterotrophically in fed-batch 50-600-L fermenters at 36A degrees C, on aerated and mixed nutrient solution with urea as a nitrogen and glucose as a carbon and energy source. Cell density increased from the initial value 6.25 to 117.18 g DW L-1 in 32 h in the fermenter 50 L at a mean growth rate 3.52 g DW L-1 h(-1). The DW increase in the fermenter 200 L was from 7.25 to 94.82 g DW L-1 in 26.5 h at a mean growth rate 3.37 g DW L-1 h(-1). Mean specific growth rate mu was about 0.1 h(-1) in the both fermenters, if nutrients and oxygen were adequately supplied. The DW increase in the fermenter 600 L was from 0.8 to 81.6 g DW L-1 in 66.5 h at a mean growth rate 1.22 g DW L-1 h(-1) and mu = 0.07 h(-1). A limitation of the cell growth rate in 600 L fermenter caused by a low dissolved oxygen concentration above cell densities higher than 10 g DW L-1) occurred. Specific growth rate decreased approximately linearly with increasing glucose concentration (25-80 g glucose L-1) at the beginning of cultivation and decreased with the time of cultivation. The cell yield was 0.55-0.69 g DW (g glucose)(-1). The content of proteins, beta-carotene, and chlorophylls in the cells steadily increased and starch content decreased, by keeping aerated and mixed culture another 12 h in fermenter after the cell growth was stopped due to glucose deficiency (en)
Title
  • Production of high-density Chlorella culture grown in fermenters
  • Production of high-density Chlorella culture grown in fermenters (en)
skos:prefLabel
  • Production of high-density Chlorella culture grown in fermenters
  • Production of high-density Chlorella culture grown in fermenters (en)
skos:notation
  • RIV/61388971:_____/12:00388567!RIV13-AV0-61388971
http://linked.open...avai/predkladatel
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • I, P(OE09025)
http://linked.open...iv/cisloPeriodika
  • 1
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
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http://linked.open...titaPredkladatele
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  • 162468
http://linked.open...ai/riv/idVysledku
  • RIV/61388971:_____/12:00388567
http://linked.open...riv/jazykVysledku
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  • Chlorella vulgaris; Heterotrophic culture; Fed-batch (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [3E7B82239C92]
http://linked.open...i/riv/nazevZdroje
  • Journal of Applied Phycology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
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http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 24
http://linked.open...iv/tvurceVysledku
  • Doucha, Jiří
  • Lívanský, Karel
http://linked.open...ain/vavai/riv/wos
  • 000299122200004
issn
  • 0921-8971
number of pages
http://bibframe.org/vocab/doi
  • 10.1007/s10811-010-9643-2
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