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| Description
| - Numerous Delta 12-, Delta 15- and multifunctional membrane fatty acid desaturases (FADs) have been identified in fungi, revealing great variability in the enzymatic specificities of FADs involved in biosynthesis of polyunsaturated fatty acids (PUFAs). Here, we report gene isolation and characterization of novel Delta 12/Delta 15- and Delta 15-FADs named CpFad2 and CpFad3, respectively, from the opportunistic pathogenic yeast Candida parapsilosis. Overexpression of CpFad3 in Saccharomyces cerevisiae strains supplemented with linoleic acid (Delta 9,Delta 12-18:2) and hexadecadienoic acid (Delta 9,Delta 12-16:2) leads to accumulation of Delta 15-PUFAs, i.e., alpha-linolenic acid (Delta 9,Delta 12,Delta 15-18:3) and hexadecatrienoic acid with an unusual terminal double bond (Delta 9,Delta 12,Delta 15-16:3). CpFad2 produces a range of Delta 12- and Delta 15-PUFAs. The major products of CpFad2 are linoleic and hexadecadienoic acid (Delta 9,Delta 12-16:2), accompanied by alpha-linolenic acid and hexadecatrienoic acid (Delta 9,Delta 12,Delta 15-16:3). Using GC/MS analysis of trimethylsilyl derivatives, we identified ricinoleic acid (12-hydroxy-9-octadecenoic acid) as an additional product of CpFad2. These results demonstrate that CpFAD2 is a multifunctional FAD and indicate that detailed analysis of fatty acid derivatives might uncover a range of enzymatic selectivities in other Delta 12-FADs from budding yeasts (Ascomycota: Saccharomycotina).
- Numerous Delta 12-, Delta 15- and multifunctional membrane fatty acid desaturases (FADs) have been identified in fungi, revealing great variability in the enzymatic specificities of FADs involved in biosynthesis of polyunsaturated fatty acids (PUFAs). Here, we report gene isolation and characterization of novel Delta 12/Delta 15- and Delta 15-FADs named CpFad2 and CpFad3, respectively, from the opportunistic pathogenic yeast Candida parapsilosis. Overexpression of CpFad3 in Saccharomyces cerevisiae strains supplemented with linoleic acid (Delta 9,Delta 12-18:2) and hexadecadienoic acid (Delta 9,Delta 12-16:2) leads to accumulation of Delta 15-PUFAs, i.e., alpha-linolenic acid (Delta 9,Delta 12,Delta 15-18:3) and hexadecatrienoic acid with an unusual terminal double bond (Delta 9,Delta 12,Delta 15-16:3). CpFad2 produces a range of Delta 12- and Delta 15-PUFAs. The major products of CpFad2 are linoleic and hexadecadienoic acid (Delta 9,Delta 12-16:2), accompanied by alpha-linolenic acid and hexadecatrienoic acid (Delta 9,Delta 12,Delta 15-16:3). Using GC/MS analysis of trimethylsilyl derivatives, we identified ricinoleic acid (12-hydroxy-9-octadecenoic acid) as an additional product of CpFad2. These results demonstrate that CpFAD2 is a multifunctional FAD and indicate that detailed analysis of fatty acid derivatives might uncover a range of enzymatic selectivities in other Delta 12-FADs from budding yeasts (Ascomycota: Saccharomycotina). (en)
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| Title
| - Delta 12-Fatty Acid Desaturase from Candida parapsilosis Is a Multifunctional Desaturase Producing a Range of Polyunsaturated and Hydroxylated Fatty Acids
- Delta 12-Fatty Acid Desaturase from Candida parapsilosis Is a Multifunctional Desaturase Producing a Range of Polyunsaturated and Hydroxylated Fatty Acids (en)
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| skos:prefLabel
| - Delta 12-Fatty Acid Desaturase from Candida parapsilosis Is a Multifunctional Desaturase Producing a Range of Polyunsaturated and Hydroxylated Fatty Acids
- Delta 12-Fatty Acid Desaturase from Candida parapsilosis Is a Multifunctional Desaturase Producing a Range of Polyunsaturated and Hydroxylated Fatty Acids (en)
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| skos:notation
| - RIV/61388963:_____/14:00427919!RIV15-TA0-61388963
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61388963:_____/14:00427919
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - yeast Saccharomyces cerevisiae; omega-3 fatty acid desaturase; heterologous expression (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Pichová, Iva
- Sychrová, Hana
- Matoušková, Petra
- Hrušková-Heidingsfeldová, Olga
- Buček, Aleš
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| http://linked.open...ain/vavai/riv/wos
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| issn
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| number of pages
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| http://bibframe.org/vocab/doi
| - 10.1371/journal.pone.0093322
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