About: Raman Microspectroscopy of the Yeast Vacuoles

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In the present work, real ability of a confocal Raman microspectroscopy to monitor chemical composition of the vacuoles within living yeast cells was investigated and critically assessed. Simple, economical, and practical protocols of the yeast immobilization suitable for less laborious, high-throughput, and spatially resolved Raman measurements were tested for their possible impacts on physiological states and viability of the cells. We have demonstrated that, acquiring Raman spectra from statistically sound sets of immobilized cells and employing advanced multivariate methods for spectral analysis, the chemical composition of the yeast vacuoles can be reliably studied. The most easily and accurately quantifiable seems to be the concentration of polyphosphates which can be unambiguously identified due to unmistakable Raman features. Our approach can be useful for routine, label-free, and noninvasive monitoring of the chemical composition of the vacuoles of living yeasts exposed to various stress factors, the information important in biomedical research of pathogens. In the present work, real ability of a confocal Raman microspectroscopy to monitor chemical composition of the vacuoles within living yeast cells was investigated and critically assessed. Simple, economical, and practical protocols of the yeast immobilization suitable for less laborious, high-throughput, and spatially resolved Raman measurements were tested for their possible impacts on physiological states and viability of the cells. We have demonstrated that, acquiring Raman spectra from statistically sound sets of immobilized cells and employing advanced multivariate methods for spectral analysis, the chemical composition of the yeast vacuoles can be reliably studied. The most easily and accurately quantifiable seems to be the concentration of polyphosphates which can be unambiguously identified due to unmistakable Raman features. Our approach can be useful for routine, label-free, and noninvasive monitoring of the chemical composition of the vacuoles of living yeasts exposed to various stress factors, the information important in biomedical research of pathogens. (en)
Title	Raman Microspectroscopy of the Yeast Vacuoles Raman Microspectroscopy of the Yeast Vacuoles (en)
skos:prefLabel	Raman Microspectroscopy of the Yeast Vacuoles Raman Microspectroscopy of the Yeast Vacuoles (en)
skos:notation	RIV/61388963:_____/12:00378875!RIV13-AV0-61388963
http://linked.open...avai/predkladatel	Ústav organické chemie a biochemie AV ČR, v. v. i.
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA310/09/1945), P(GAP208/10/0376), Z(AV0Z40550506), Z(MSM0021620835)
http://linked.open...iv/cisloPeriodika	5-6
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Pichová, Iva Hrušková, Olga Bednárová, Lucie Bauerová, Václava
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav organické chemie a biochemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	164020
http://linked.open...ai/riv/idVysledku	RIV/61388963:_____/12:00378875
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Raman microspectroscopy; living cell; yeast; vacuole; chemical composition; polyphospate; Candida albicans (en)
http://linked.open.../riv/klicoveSlovo	Raman microspectroscopy chemical composition yeast vacuole polyphospate Candida albicans living cell
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[502B00708886]
http://linked.open...i/riv/nazevZdroje	Spectroscopy
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Role of aspartic proteinases in nutrient acquisition and starvation of pathogenic yeasts Candida albicans and Candida parapsilosis Interaction of antibacterial peptides with the model membranes and possibility of the prediction of their biological activity
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	27
http://linked.open...iv/tvurceVysledku	Bednárová, Lucie Pichová, Iva Hrušková, Olga Bauerová, Václava Mojzeš, P. Palacký, J.
http://linked.open...ain/vavai/riv/wos	000306505500029
http://linked.open...n/vavai/riv/zamer	Regulace biologických procesů: Chemické modulátory vybraných systémů významných pro medicínu a zemědělství Fyzika molekulárních, makromolekulárních a biologických systémů
issn	0712-4813
number of pages	5 (xsd:int)
http://bibframe.org/vocab/doi	10.1155/2012/746597
is http://linked.open...avai/riv/vysledek of	Raman Microspectroscopy of the Yeast Vacuoles

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