About: Surface sticking and lateral diffusion of lipids in supported bilayers

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Difůze fluorescenčně značených lipidů byla sledována pomocí „Z-scans fluorescnční korelační spektroskopie“ a dvou ohniskové fluorescenční korelační spektroskopie. Z naměřených dat je patrné ulpívání molekul lipidu na povrchu. Tento proces je velmi pomalý, nicméně jeho zanedbání vede ke zkresleným hodnotám difůzního koeficientu. (cs) The diffusion of fluorescently labeled lipids in supported bilayers is studied using two different methods: Z-scan fluorescence correlation spectroscopy (z-scan FCS) and two-focus fluorescence correlation spectroscopy (2f-FCS). It is found that the data can be fitted consistently only when taking into account partial sticking of the labeled lipids to the supporting glass surface. A kinetic reaction-diffusion model is developed and applied to the data. We find a very slow sticking rate which, however, when neglected, leads to strongly varying estimates of the free diffusion coefficient. The study reveals a strong sensitivity of FCS on even slight binding/unbinding kinetics of the labeled molecules, which has significance for related diffusion measurements in cellular lipid membranes. The diffusion of fluorescently labeled lipids in supported bilayers is studied using two different methods: Z-scan fluorescence correlation spectroscopy (z-scan FCS) and two-focus fluorescence correlation spectroscopy (2f-FCS). It is found that the data can be fitted consistently only when taking into account partial sticking of the labeled lipids to the supporting glass surface. A kinetic reaction-diffusion model is developed and applied to the data. We find a very slow sticking rate which, however, when neglected, leads to strongly varying estimates of the free diffusion coefficient. The study reveals a strong sensitivity of FCS on even slight binding/unbinding kinetics of the labeled molecules, which has significance for related diffusion measurements in cellular lipid membranes. (en)
Title	Surface sticking and lateral diffusion of lipids in supported bilayers Povrchové ulpívání a laterální difúze lipidů v podporovaných dvojvrstvách (cs) Surface sticking and lateral diffusion of lipids in supported bilayers (en)
skos:prefLabel	Surface sticking and lateral diffusion of lipids in supported bilayers Povrchové ulpívání a laterální difúze lipidů v podporovaných dvojvrstvách (cs) Surface sticking and lateral diffusion of lipids in supported bilayers (en)
skos:notation	RIV/61388955:_____/06:00043204!RIV07-AV0-61388955
http://linked.open.../vavai/riv/strany	9339;9344
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/05/2308), Z(AV0Z40400503)
http://linked.open...iv/cisloPeriodika	22
http://linked.open...vai/riv/dodaniDat	2007
http://linked.open...aciTvurceVysledku	Hof, Martin Benda, Aleš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav fyzikální chemie J. Heyrovského AV ČR, v.v.i.
http://linked.open...dnocenehoVysledku	502557
http://linked.open...ai/riv/idVysledku	RIV/61388955:_____/06:00043204
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	fluorescence-correlation spectroscopy; atomic-force microscopy; total internal-reflection (en)
http://linked.open.../riv/klicoveSlovo	atomic-force microscopy fluorescence-correlation spectroscopy total internal-reflection
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[F6675E532E4A]
http://linked.open...i/riv/nazevZdroje	Langmuir
http://linked.open...in/vavai/riv/obor	CF
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Understanding, control, and preparation of self-assembling biomembrane systems at interfaces
http://linked.open...UplatneniVysledku	2006
http://linked.open...v/svazekPeriodika	22
http://linked.open...iv/tvurceVysledku	Hof, Martin Benda, Aleš Enderlain, J. Dertinger, T. von der Hocht, I.
http://linked.open...n/vavai/riv/zamer	Struktura, reaktivita a dynamika molekulárních a biomolekulárních systémů: teorie, experiment, aplikace
issn	0743-7463
number of pages	6 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Surface sticking and lateral diffusion of lipids in supported bilayers Surface sticking and lateral diffusion of lipids in supported bilayers

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