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  • Although there exist a number of methods, such as NMR, X-ray, e.g., which explore the hydration of phospholipid bilayers, the solvent relaxation (SR) method has the advantage of simple instrumentation, easy data treatment and possibility of measuring fully hydrated samples. The main information gained from SR by the analysis of recorded %22time-resolved emission spectra%22 (TRES) is microviscosity and micro-polarity of the dye microenvironment. Based on these parameters, one can draw conclusions about water structure in the bilayer. In this review, we focus on physical background of this method, on all the procedures that are needed in order to obtain relevant parameters, and on the requirements on the fluorescence dyes. Furthermore. a few recent applications (the effect of curvature, binding of antibacterial peptides and phase transition) illustrating the versatility of this method are mentioned. Moreover, limitations and potential problems are discussed.
  • Although there exist a number of methods, such as NMR, X-ray, e.g., which explore the hydration of phospholipid bilayers, the solvent relaxation (SR) method has the advantage of simple instrumentation, easy data treatment and possibility of measuring fully hydrated samples. The main information gained from SR by the analysis of recorded %22time-resolved emission spectra%22 (TRES) is microviscosity and micro-polarity of the dye microenvironment. Based on these parameters, one can draw conclusions about water structure in the bilayer. In this review, we focus on physical background of this method, on all the procedures that are needed in order to obtain relevant parameters, and on the requirements on the fluorescence dyes. Furthermore. a few recent applications (the effect of curvature, binding of antibacterial peptides and phase transition) illustrating the versatility of this method are mentioned. Moreover, limitations and potential problems are discussed. (en)
  • Tato práce pojednává o reakcích rozpouštědla ve fosfolipidových dvojvrstvách. Popisuje její principy a nové aplikace. (cs)
Title
  • Solvent relaxation in phospholipid bilayers: Principles and recent applications
  • Solvent relaxation in phospholipid bilayers: Principles and recent applications (en)
  • Reakce rozpouštědla ve fosfolipidových dvjvrstvách: Nové principy a aplikace (cs)
skos:prefLabel
  • Solvent relaxation in phospholipid bilayers: Principles and recent applications
  • Solvent relaxation in phospholipid bilayers: Principles and recent applications (en)
  • Reakce rozpouštědla ve fosfolipidových dvjvrstvách: Nové principy a aplikace (cs)
skos:notation
  • RIV/61388955:_____/05:00030741!RIV06-AV0-61388955
http://linked.open.../vavai/riv/strany
  • 883;894
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA203/05/2308), P(IAA400400503), Z(AV0Z40400503)
http://linked.open...iv/cisloPeriodika
  • 6
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 543465
http://linked.open...ai/riv/idVysledku
  • RIV/61388955:_____/05:00030741
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • time-resolved emission spectroscopy; solvent relaxation; membrane curvature; antibacterial peptides (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [69D2A3A49C66]
http://linked.open...i/riv/nazevZdroje
  • Journal of Fluorescence
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 15
http://linked.open...iv/tvurceVysledku
  • Hof, Martin
  • Jurkiewicz, Piotr
  • Sýkora, Jan
  • Olžyńska, Agnieszka
  • Humpolíčková, Jana
http://linked.open...n/vavai/riv/zamer
issn
  • 1053-0509
number of pages
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