About: TCSPC upgrade of a confocal FCS microscope

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Rozšíření konfokálního FCS mikroskopu Carl Zeiss Confocor 1 pomocí pulsních laserů, rychlejšího detektoru a čítací karty PicoQuant TimeHarp 200 umožňuje zaznamenat kompletní dynamiku detekované fluorescence – časové fluktuace intensity a vyhasínání fluorescence. Kombinace těchto informací umožňuje tzv. časově rozlišenou fluorescenční korelační analýzu. Užitečnost této analýzy je ukázána na modelovém měření výměny barviva mezi dvěma populace jednovrstvých vesikul. (cs) We extended the measurement capabilities of the Carl Zeiss ConfoCor 1 FCS microscope by (a) using pulsed picosecond diode lasers instead of a continuous wave (CW) laser excitation, (b) introducing a fast single photon avalanche diode detector, and (c) exploiting the capabilities of the PicoQuant TimeHarp 200 board. When the time-tagged time-resolved (TTTR) mode of the TimeHarp is utilized, the complete fluorescence dynamics are recorded. That is, the time-evolution of the fluctuations and the fluorescence decay kinetics are captured simultaneously. Recording individual photon events (without on-the-fly data reduction like in hardware correlators) preserves the full information content of the measurement for virtually unlimited data analysis tasks and provides a much more detailed view of processes happening in the detection volume. We extended the measurement capabilities of the Carl Zeiss ConfoCor 1 FCS microscope by (a) using pulsed picosecond diode lasers instead of a continuous wave (CW) laser excitation, (b) introducing a fast single photon avalanche diode detector, and (c) exploiting the capabilities of the PicoQuant TimeHarp 200 board. When the time-tagged time-resolved (TTTR) mode of the TimeHarp is utilized, the complete fluorescence dynamics are recorded. That is, the time-evolution of the fluctuations and the fluorescence decay kinetics are captured simultaneously. Recording individual photon events (without on-the-fly data reduction like in hardware correlators) preserves the full information content of the measurement for virtually unlimited data analysis tasks and provides a much more detailed view of processes happening in the detection volume. (en)
Title	TCSPC upgrade of a confocal FCS microscope Rozšíření konfokálního FCS mikroskopu časově korelovaným čítáním fotonů (cs) TCSPC upgrade of a confocal FCS microscope (en)
skos:prefLabel	TCSPC upgrade of a confocal FCS microscope Rozšíření konfokálního FCS mikroskopu časově korelovaným čítáním fotonů (cs) TCSPC upgrade of a confocal FCS microscope (en)
skos:notation	RIV/61388955:_____/05:00000620!RIV06-AV0-61388955
http://linked.open.../vavai/riv/strany	033106;1;033106;4
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1ET400400413), P(GA203/05/2308), Z(AV0Z40400503)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2006
http://linked.open...aciTvurceVysledku	Hof, Martin Benda, Aleš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav fyzikální chemie J. Heyrovského AV ČR, v.v.i.
http://linked.open...dnocenehoVysledku	546010
http://linked.open...ai/riv/idVysledku	RIV/61388955:_____/05:00000620
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	fluorescence correlation spectroscopy; photon; TCSPC (en)
http://linked.open.../riv/klicoveSlovo	photon TCSPC fluorescence correlation spectroscopy
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[C97860823E2A]
http://linked.open...i/riv/nazevZdroje	Review of Scientific Instruments
http://linked.open...in/vavai/riv/obor	CF
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Development of a program environment for mathematic simulations and predictions in catalysis and electrocatalysis Understanding, control, and preparation of self-assembling biomembrane systems at interfaces
http://linked.open...UplatneniVysledku	2005
http://linked.open...v/svazekPeriodika	76
http://linked.open...iv/tvurceVysledku	Hof, Martin Benda, Aleš Kapusta, P. Wahl, M. Erdmann, R. Patting, M.
http://linked.open...n/vavai/riv/zamer	Struktura, reaktivita a dynamika molekulárních a biomolekulárních systémů: teorie, experiment, aplikace
issn	0034-6748
number of pages	4 (xsd:int)
is http://linked.open...avai/riv/vysledek of	TCSPC upgrade of a confocal FCS microscope

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