About: Synthesis of hapten and conjugates of coumestrol and development of immunoassay

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About: Synthesis of hapten and conjugates of coumestrol and development of immunoassay Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	3-O-Carboxymethylcoumestrol was prepared as the hapten for immunoassay by a partial alkylation of coumestrol with ethyl chloroacetate in acetone alkalized with potassium carbonate. 3-O-Ethoxycarbonylmethylcoumestrol was separated by column chromatographyand finally was hydrolyzed with formic acid. 1H and 13C NMR data (APT, COSY, HMQC, and HMBC) revealed that the reaction was regioselective, as 3-O-ethoxycarboxymethylcoumestrol was the only monosubstituted derivative. The hapten was then conjugated to b ovine serum albumin and used for immunization of rabbits. A radioimmunoassay (RIA) system was established based on the polyclonal antiserum and a 125I-labeled hapten-tyrosine methyl ester conjugate as the radioligand. Parameters of the RIA: sensitivity: 12 pg per tube, 50% intercept: 140 pg per tube, working range: 20-4000 pg per tube. The cross-reactivity of a panel isoflavonoid and lignan phytoestrogens was either negligible (e.g. formononetin 0.07%; biochanin A 0.06%) or not detectable at all. The ma 3-O-Carboxymethylcoumestrol was prepared as the hapten for immunoassay by a partial alkylation of coumestrol with ethyl chloroacetate in acetone alkalized with potassium carbonate. 3-O-Ethoxycarbonylmethylcoumestrol was separated by column chromatographyand finally was hydrolyzed with formic acid. 1H and 13C NMR data (APT, COSY, HMQC, and HMBC) revealed that the reaction was regioselective, as 3-O-ethoxycarboxymethylcoumestrol was the only monosubstituted derivative. The hapten was then conjugated to b ovine serum albumin and used for immunization of rabbits. A radioimmunoassay (RIA) system was established based on the polyclonal antiserum and a 125I-labeled hapten-tyrosine methyl ester conjugate as the radioligand. Parameters of the RIA: sensitivity: 12 pg per tube, 50% intercept: 140 pg per tube, working range: 20-4000 pg per tube. The cross-reactivity of a panel isoflavonoid and lignan phytoestrogens was either negligible (e.g. formononetin 0.07%; biochanin A 0.06%) or not detectable at all. The ma (en)
Title	Synthesis of hapten and conjugates of coumestrol and development of immunoassay Synthesis of hapten and conjugates of coumestrol and development of immunoassay (en)
skos:prefLabel	Synthesis of hapten and conjugates of coumestrol and development of immunoassay Synthesis of hapten and conjugates of coumestrol and development of immunoassay (en)
skos:notation	RIV/60461373:22640/03:00008946!RIV/2004/GA0/226404/N
http://linked.open.../vavai/riv/strany	1147-1155
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA523/00/0567), P(GA525/03/0352)
http://linked.open...iv/cisloPeriodika	68
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Šiklová-Vítková, Michaela Kleinová, Tereza Štursa, Jan Moravcová, Jitka Dvořáková, Hana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Centrální laboratoře
http://linked.open...dnocenehoVysledku	630049
http://linked.open...ai/riv/idVysledku	RIV/60461373:22640/03:00008946
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Steroid, Phytoestrogen, Coumestrol, Immunoassay, Alfalfa (en)
http://linked.open.../riv/klicoveSlovo	Immunoassay Steroid Alfalfa Coumestrol Phytoestrogen
http://linked.open...odStatuVydavatele	BE - Belgické království
http://linked.open...ontrolniKodProRIV	[EBE9C3FDCCE8]
http://linked.open...i/riv/nazevZdroje	Steroids
http://linked.open...in/vavai/riv/obor	CC
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Phytoestrogens of alfalfa as a risk factor in animal feed: occurrence, distribution, changes during ensiling Isoflavonoids in the Myrtaceae and the Rutaceae families
http://linked.open...UplatneniVysledku	2003
http://linked.open...v/svazekPeriodika	2003
http://linked.open...iv/tvurceVysledku	Lapčík, Oldřich Dvořáková, Hana Vítková, Michaela Štursa, Jan Moravcová, Jitka Klejdus, B. Kleinová, Tereza
issn	0039-128X
number of pages	9 (xsd:int)
http://localhost/t...ganizacniJednotka	22640

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