About: Effect of cross-linking method on the activity of spray-dried chitosan microparticles with immobilized laccase

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About: Effect of cross-linking method on the activity of spray-dried chitosan microparticles with immobilized laccase Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The effect of processing method on the properties of cross-linked chitosan microparticles and on the enzymatic activity of laccase immobilized in the particles has been investigated. Chitosan has been cross-linked by tri-polyphosphate (TPP) using two methods ? the so called ex situ cross-linking whereby the solutions of chitosan, TPP and the enzyme have been pre-mixed and spray-dried by a standard two-fluid kinetic nozzle, and a novel in situ cross-linking method, whereby the solutions have been contacted at the tip of a three-fluid nozzle and cross-linking occurred within a drying droplet. The influence of the cross-linking method on the particle size and morphology, surface charge, and swelling ratio has been determined. The enzymatic activity of laccase toward the oxidation of a chromophore substrate (ABTS) has been systematically investigated and found to be superior in particles produced by the in situ cross-linking method. The effect of processing method on the properties of cross-linked chitosan microparticles and on the enzymatic activity of laccase immobilized in the particles has been investigated. Chitosan has been cross-linked by tri-polyphosphate (TPP) using two methods ? the so called ex situ cross-linking whereby the solutions of chitosan, TPP and the enzyme have been pre-mixed and spray-dried by a standard two-fluid kinetic nozzle, and a novel in situ cross-linking method, whereby the solutions have been contacted at the tip of a three-fluid nozzle and cross-linking occurred within a drying droplet. The influence of the cross-linking method on the particle size and morphology, surface charge, and swelling ratio has been determined. The enzymatic activity of laccase toward the oxidation of a chromophore substrate (ABTS) has been systematically investigated and found to be superior in particles produced by the in situ cross-linking method. (en)
Title	Effect of cross-linking method on the activity of spray-dried chitosan microparticles with immobilized laccase Effect of cross-linking method on the activity of spray-dried chitosan microparticles with immobilized laccase (en)
skos:prefLabel	Effect of cross-linking method on the activity of spray-dried chitosan microparticles with immobilized laccase Effect of cross-linking method on the activity of spray-dried chitosan microparticles with immobilized laccase (en)
skos:notation	RIV/60461373:22340/13:43895409!RIV14-MSM-22340___
http://linked.open...avai/predkladatel	Fakulta chemicko-inženýrská
http://linked.open...avai/riv/aktivita	P S
http://linked.open...avai/riv/aktivity	P(MEB061108), S
http://linked.open...iv/cisloPeriodika	C4
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Štěpánek, František Tokárová, Viola Kašpar, Ondřej
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta chemicko-inženýrská
http://linked.open...dnocenehoVysledku	71437
http://linked.open...ai/riv/idVysledku	RIV/60461373:22340/13:43895409
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Diffusion; Swelling; Kinetics; Enzyme; Encapsulation; Spray drying (en)
http://linked.open.../riv/klicoveSlovo	Enzyme Spray drying Encapsulation Diffusion Swelling Kinetics
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[02F8B777902D]
http://linked.open...i/riv/nazevZdroje	Food and Bioproducts Processing
http://linked.open...in/vavai/riv/obor	CI
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Development of chitosan based biomedical polymers with antibacterial properties
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	91
http://linked.open...iv/tvurceVysledku	Tokárová, Viola Štěpánek, František Kašpar, Ondřej Gübitz, Georg Nyanhongo, Gibson S.
http://linked.open...ain/vavai/riv/wos	000328009100027
issn	0960-3085
number of pages	14 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.fbp.2013.06.001
http://localhost/t...ganizacniJednotka	22340

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