About: On-line Monitoring of PHA Cellular Content in Pseudomonas putida Cultures Using Radio-frequency Impedance

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Radio-frequency impedance based biomass measurements represent a relatively accurate and reliable method for on-line quantification of viable cellular biomass. For the living biomass concentration determination, a linear correlation between biomass concentration and capacitance is generally assumed. However, this assumption is not always valid as e.g. the presence of intracellular lipid inclusions may violate the assumption of linear correlation. Therefore, the main objective of this study was to examine the potential of the sensor for on-line in-situ monitoring of intracellular biopolymer content (PHA) in bacterial cells. It was experimentally confirmed that the loss of linearity between dry cell weight and capacitance takes place also in the case of the Pseudomonas putida culture. The resulting nonlinear behaviour can be used for the design of a novel biopolymer content estimator based on the capacitance and offgas composition measurements. Radio-frequency impedance based biomass measurements represent a relatively accurate and reliable method for on-line quantification of viable cellular biomass. For the living biomass concentration determination, a linear correlation between biomass concentration and capacitance is generally assumed. However, this assumption is not always valid as e.g. the presence of intracellular lipid inclusions may violate the assumption of linear correlation. Therefore, the main objective of this study was to examine the potential of the sensor for on-line in-situ monitoring of intracellular biopolymer content (PHA) in bacterial cells. It was experimentally confirmed that the loss of linearity between dry cell weight and capacitance takes place also in the case of the Pseudomonas putida culture. The resulting nonlinear behaviour can be used for the design of a novel biopolymer content estimator based on the capacitance and offgas composition measurements. (en)
Title	On-line Monitoring of PHA Cellular Content in Pseudomonas putida Cultures Using Radio-frequency Impedance On-line Monitoring of PHA Cellular Content in Pseudomonas putida Cultures Using Radio-frequency Impedance (en)
skos:prefLabel	On-line Monitoring of PHA Cellular Content in Pseudomonas putida Cultures Using Radio-frequency Impedance On-line Monitoring of PHA Cellular Content in Pseudomonas putida Cultures Using Radio-frequency Impedance (en)
skos:notation	RIV/60461373:22340/09:00022071!RIV10-MSM-22340___
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM6046137306)
http://linked.open...iv/cisloPeriodika	S1
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Jurníček, Vladimír Hrnčiřík, Pavel Náhlík, Jan Vovsík, Jaroslav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta chemicko-inženýrská
http://linked.open...dnocenehoVysledku	331676
http://linked.open...ai/riv/idVysledku	RIV/60461373:22340/09:00022071
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	on-line monitoring; biopolymer production; fed-batch cultivation; radio-frequency impedance measurement (en)
http://linked.open.../riv/klicoveSlovo	radio-frequency impedance measurement biopolymer production fed-batch cultivation on-line monitoring
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[1B394ADE8FA2]
http://linked.open...i/riv/nazevZdroje	New Biotechnology
http://linked.open...in/vavai/riv/obor	JD
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	25
http://linked.open...iv/tvurceVysledku	Hrnčiřík, Pavel Náhlík, Jan Vovsík, Jaroslav Jurníček, Vladimír
http://linked.open...n/vavai/riv/zamer	Reakčně-transportní chemicko-inženýrské systémy a jejich dynamické chování, modelování a řízení chemických a biotechnologických procesů
issn	1871-6784
number of pages	2 (xsd:int)
http://localhost/t...ganizacniJednotka	22340
is http://linked.open...avai/riv/vysledek of	On-line Monitoring of PHA Cellular Content in Pseudomonas putida Cultures Using Radio-frequency Impedance On-line Monitoring of PHA Cellular Content in Pseudomonas putida Cultures Using Radio-frequency Impedance On-line Monitoring of PHA Cellular Content in Pseudomonas putida Cultures Using Radio-frequency Impedance On-line Monitoring of PHA Cellular Content in Pseudomonas putida Cultures Using Radio-frequency Impedance

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