About: HETEROGENEOUS IMMUNOASSAY IN POLYSTYRENE MICROCHIPS

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Práce je zaměřena na vývoj mikrofluidních zařízení pro kvantitativní stanovení IgG. Zařízení je vyrobeno technikou mikroobrábění. Experimentální data dokazují, že kvantitativní detekce IgG v navrženém multikanálovém mikročipu je možná během několika minut. (cs) The work is focused on development of microfluidic polystyrene chips for detection of biological agents. The microfluidic chips are constructed with the use of the micro-milling technology. The interaction among the immobilized bio-molecules and the complementary molecules from an analyte is the principle of the microfluidic chip. Here, the immobilized Protein A (PA) binds the complementary human immunoglobulin G (IgG) from the liquid sample and the protein-protein complex is formed. Because the immunoglobulins are fluorescently labeled, the observed fluorescence signal corresponds with the concentration of the biological macromolecule in the sample. Specificity of the interaction PA - human IgG is tested in blind experiments. Bovine serum albumin (BSA) is used as the blocking agent and Tris buffer with detergent Tween disintegrates the non-specific bonds. The experimental results demonstrate that the immunoglobulins can be quantitatively detected in the proposed multichannel microchip within few The work is focused on development of microfluidic polystyrene chips for detection of biological agents. The microfluidic chips are constructed with the use of the micro-milling technology. The interaction among the immobilized bio-molecules and the complementary molecules from an analyte is the principle of the microfluidic chip. Here, the immobilized Protein A (PA) binds the complementary human immunoglobulin G (IgG) from the liquid sample and the protein-protein complex is formed. Because the immunoglobulins are fluorescently labeled, the observed fluorescence signal corresponds with the concentration of the biological macromolecule in the sample. Specificity of the interaction PA - human IgG is tested in blind experiments. Bovine serum albumin (BSA) is used as the blocking agent and Tris buffer with detergent Tween disintegrates the non-specific bonds. The experimental results demonstrate that the immunoglobulins can be quantitatively detected in the proposed multichannel microchip within few (en)
Title	HETEROGENEOUS IMMUNOASSAY IN POLYSTYRENE MICROCHIPS HETEROGENEOUS IMMUNOASSAY IN POLYSTYRENE MICROCHIPS (en) Heteogenní imunoanalýza v polystyrenovém mikročipu (cs)
skos:prefLabel	HETEROGENEOUS IMMUNOASSAY IN POLYSTYRENE MICROCHIPS HETEROGENEOUS IMMUNOASSAY IN POLYSTYRENE MICROCHIPS (en) Heteogenní imunoanalýza v polystyrenovém mikročipu (cs)
skos:notation	RIV/60461373:22340/06:00016656!RIV07-MSM-22340___
http://linked.open.../vavai/riv/strany	075(1-6)
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GP104/03/D006), Z(MSM6046137306)
http://linked.open...vai/riv/dodaniDat	2007
http://linked.open...aciTvurceVysledku	Marek, Miloš
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta chemicko-inženýrská
http://linked.open...dnocenehoVysledku	477615
http://linked.open...ai/riv/idVysledku	RIV/60461373:22340/06:00016656
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	heterogeneous immunoassay; antigen; antibody; microchip; microdevice; microsensor; protein A; human IgG (en)
http://linked.open.../riv/klicoveSlovo	antigen microchip antibody heterogeneous immunoassay microdevice protein A human IgG microsensor
http://linked.open...ontrolniKodProRIV	[9C70AED74EFC]
http://linked.open...v/mistoKonaniAkce	Tatranské Matliare
http://linked.open...i/riv/mistoVydani	Bratislava
http://linked.open...i/riv/nazevZdroje	33rd International Conference of SSCHE, Tatranské Matliare
http://linked.open...in/vavai/riv/obor	CI
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Study of microreactors for bioengineering application
http://linked.open...UplatneniVysledku	2006
http://linked.open...iv/tvurceVysledku	Přibyl, Michal Marek, Miloš Štěpánek, Jakub
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2006-05-22 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Reakčně-transportní chemicko-inženýrské systémy a jejich dynamické chování, modelování a řízení chemických a biotechnologických procesů
number of pages	6 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Slovak Society of Chemical Engineering
https://schema.org/isbn	80-227-2409-2
http://localhost/t...ganizacniJednotka	22340
is http://linked.open...avai/riv/vysledek of	HETEROGENEOUS IMMUNOASSAY IN POLYSTYRENE MICROCHIPS HETEROGENEOUS IMMUNOASSAY IN POLYSTYRENE MICROCHIPS

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