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rdf:type
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Description
| - Bylo vyvinuto mikrofluidní zařízení pro heterogenní imunoanalýzu na polystyrenové platformě. Metoda zatlačování drátků za teplabyla zvolena při konstrukci mikrokanálkových struktur. Imunoanalýza byla založena na systému fluorescenčně značeného lidského IgG a proteinu A. Roztoky a vzorky jsou manipulovány pomocí elektroosmotického toku. Hodnota elektroosmotického koeficientu 2,1e-8 m2/V/s byla nalezena. Matematický model byl sestaven a analyzován. (cs)
- We have developed a microfluidic device for heterogeneous immunoassay on the polystyrene platform. The wire-imprinting method has been chosen for the construction of microchannel structure of the chip. Immunoassay based on the interaction of fluorescently labeled human IgG (FITC-IgG) and Protein A (antigen) is selected as experimental system. The sample and other solutions are manipulated only by the electroosmotic mechanism. We have measured the electroosmotic mobility coefficient in the microchannels equal to mu_eo = 2.1 10-8 m2V-1s-1. Mathematical model of the microchip of the same geometry based on the transport-reaction equations, the equation of electrostatics, the momentum balances and the slip boundary conditions was also constructed and analyzed.
- We have developed a microfluidic device for heterogeneous immunoassay on the polystyrene platform. The wire-imprinting method has been chosen for the construction of microchannel structure of the chip. Immunoassay based on the interaction of fluorescently labeled human IgG (FITC-IgG) and Protein A (antigen) is selected as experimental system. The sample and other solutions are manipulated only by the electroosmotic mechanism. We have measured the electroosmotic mobility coefficient in the microchannels equal to mu_eo = 2.1 10-8 m2V-1s-1. Mathematical model of the microchip of the same geometry based on the transport-reaction equations, the equation of electrostatics, the momentum balances and the slip boundary conditions was also constructed and analyzed. (en)
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Title
| - Development of Microfluidic Device for Heterogeneous Immunoassay on Polystyrene Platform with Electroosmotic Control
- Development of Microfluidic Device for Heterogeneous Immunoassay on Polystyrene Platform with Electroosmotic Control (en)
- Vývoj mikrofluidního zařízení pro heterogenní imunoanalýzu na polystyrenové platformě s elektroosmotickým řízením (cs)
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skos:prefLabel
| - Development of Microfluidic Device for Heterogeneous Immunoassay on Polystyrene Platform with Electroosmotic Control
- Development of Microfluidic Device for Heterogeneous Immunoassay on Polystyrene Platform with Electroosmotic Control (en)
- Vývoj mikrofluidního zařízení pro heterogenní imunoanalýzu na polystyrenové platformě s elektroosmotickým řízením (cs)
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skos:notation
| - RIV/60461373:22340/05:00015254!RIV06-GA0-22340___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(GA104/04/1442), P(GP104/03/D006), Z(MSM6046137306)
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/60461373:22340/05:00015254
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - heterogeneous immunoassay; electroosmosis; microchip; microdevice (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...iv/tvurceVysledku
| - Šnita, Dalimil
- Přibyl, Michal
- Marek, Miloš
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http://linked.open...n/vavai/riv/zamer
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http://localhost/t...ganizacniJednotka
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