About: Development of Microfluidic Device for Heterogeneous Immunoassay on Polystyrene Platform with Electroosmotic Control     Goto   Sponge   NotDistinct   Permalink

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  • Bylo vyvinuto mikrofluidní zařízení pro heterogenní imunoanalýzu na polystyrenové platformě. Metoda zatlačování drátků za teplabyla zvolena při konstrukci mikrokanálkových struktur. Imunoanalýza byla založena na systému fluorescenčně značeného lidského IgG a proteinu A. Roztoky a vzorky jsou manipulovány pomocí elektroosmotického toku. Hodnota elektroosmotického koeficientu 2,1e-8 m2/V/s byla nalezena. Matematický model byl sestaven a analyzován. (cs)
  • We have developed a microfluidic device for heterogeneous immunoassay on the polystyrene platform. The wire-imprinting method has been chosen for the construction of microchannel structure of the chip. Immunoassay based on the interaction of fluorescently labeled human IgG (FITC-IgG) and Protein A (antigen) is selected as experimental system. The sample and other solutions are manipulated only by the electroosmotic mechanism. We have measured the electroosmotic mobility coefficient in the microchannels equal to mu_eo = 2.1 10-8 m2V-1s-1. Mathematical model of the microchip of the same geometry based on the transport-reaction equations, the equation of electrostatics, the momentum balances and the slip boundary conditions was also constructed and analyzed.
  • We have developed a microfluidic device for heterogeneous immunoassay on the polystyrene platform. The wire-imprinting method has been chosen for the construction of microchannel structure of the chip. Immunoassay based on the interaction of fluorescently labeled human IgG (FITC-IgG) and Protein A (antigen) is selected as experimental system. The sample and other solutions are manipulated only by the electroosmotic mechanism. We have measured the electroosmotic mobility coefficient in the microchannels equal to mu_eo = 2.1 10-8 m2V-1s-1. Mathematical model of the microchip of the same geometry based on the transport-reaction equations, the equation of electrostatics, the momentum balances and the slip boundary conditions was also constructed and analyzed. (en)
Title
  • Development of Microfluidic Device for Heterogeneous Immunoassay on Polystyrene Platform with Electroosmotic Control
  • Development of Microfluidic Device for Heterogeneous Immunoassay on Polystyrene Platform with Electroosmotic Control (en)
  • Vývoj mikrofluidního zařízení pro heterogenní imunoanalýzu na polystyrenové platformě s elektroosmotickým řízením (cs)
skos:prefLabel
  • Development of Microfluidic Device for Heterogeneous Immunoassay on Polystyrene Platform with Electroosmotic Control
  • Development of Microfluidic Device for Heterogeneous Immunoassay on Polystyrene Platform with Electroosmotic Control (en)
  • Vývoj mikrofluidního zařízení pro heterogenní imunoanalýzu na polystyrenové platformě s elektroosmotickým řízením (cs)
skos:notation
  • RIV/60461373:22340/05:00015254!RIV06-GA0-22340___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA104/04/1442), P(GP104/03/D006), Z(MSM6046137306)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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  • 517779
http://linked.open...ai/riv/idVysledku
  • RIV/60461373:22340/05:00015254
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • heterogeneous immunoassay; electroosmosis; microchip; microdevice (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [5CBB966D20A1]
http://linked.open...in/vavai/riv/obor
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http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Šnita, Dalimil
  • Přibyl, Michal
  • Marek, Miloš
http://linked.open...n/vavai/riv/zamer
http://localhost/t...ganizacniJednotka
  • 22340
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