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| Description
| - Vibrational circular dichroism (VCD), electronic circular dichroism (ECD) and IR and UV-Vis absorption spectra were measured for complexes of DNA with 5, 10, 15, 20-Tetrakis(1-methylpyridinium-4-yl)porphyrin (TMPyP) at the [DNA]/[TMPyP] ratios 2/1 - 10/1. The nitrogen base and phosphate spectral regions in IR and the Soret band and DNA regions were studied in UV-Vis. The VCD spectra indicate the priority of intercalation to external minor groove binding and the saturation of the intercalating sites for the [DNA]/[TMPyP] = 4/1 - 5/1. The combination of spectroscopic results proved that all three binding modes, intercalation, minor groove binding and external binding to the phosphate backbone, take place for the ratio 3/1. It was shown that the melting point of the DNA - TMPyP complex is increased compared to pure DNA. The relative temperature stability of minor groove binding is smaller than of intercalation. The AT base-pair hydrogen bonds were disrupted at low pH for DNA - TMPyP complex contrary to
- Vibrational circular dichroism (VCD), electronic circular dichroism (ECD) and IR and UV-Vis absorption spectra were measured for complexes of DNA with 5, 10, 15, 20-Tetrakis(1-methylpyridinium-4-yl)porphyrin (TMPyP) at the [DNA]/[TMPyP] ratios 2/1 - 10/1. The nitrogen base and phosphate spectral regions in IR and the Soret band and DNA regions were studied in UV-Vis. The VCD spectra indicate the priority of intercalation to external minor groove binding and the saturation of the intercalating sites for the [DNA]/[TMPyP] = 4/1 - 5/1. The combination of spectroscopic results proved that all three binding modes, intercalation, minor groove binding and external binding to the phosphate backbone, take place for the ratio 3/1. It was shown that the melting point of the DNA - TMPyP complex is increased compared to pure DNA. The relative temperature stability of minor groove binding is smaller than of intercalation. The AT base-pair hydrogen bonds were disrupted at low pH for DNA - TMPyP complex contrary to (en)
- Komplexy DNA s 5,10,15,20-tetrakis(1-methylpyridinium-4-yl)porfyrinem (TMPyP), při poměrech [DNA]/[TMPyP] = 2/1 - 10/1, byly měřeny metodami vibračního cirkulárního dichroismu (VCD), elektronového cirkulárního dichroismu (ECD), infračervené a UV-Vis absorpční spektroskopie. Infračervenou spektroskopií byly zkoumány oblasti charakteristických vibrací dusíkových bází a fosfátové skupiny, UV-Vis spektroskopií byly zkoumány oblasti Soretova pásu a oblast absorpce DNA. Z VCD spekter bylo patrné upřednostnění interkalační vazby před vazbou do vnějšího žlábku a saturace interkalačních míst pro poměr [DNA]/[TMPyP] = 4/1 - 5/1. Z výsledků různých spektroskopických měření plyne, že při poměru 3/1 nastaly všechny tři typy vazeb, kterými jsou: interkalace, vazba do vnějšího žlábku a vnější vazba na fosfátovou páteř. Bylo prokázáno, že teplota tání komplexu DNA - TMPyP je ve srovnání s čistou DNA vyšší. Teplotní stabilita vazby TMPyP do vnějšího žlábku je menší než teplotní stabilita interkalace. Narozdíl od čisté (cs)
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| Title
| - Vibrational and Electronic Circular Dichroism and Absorption Spectral Study of the DNA - 5, 10, 15, 20-tetrakis(1-methylpyridinium-4-yl)porphyrin Interaction
- Vibračně a elektronově cirkulárně dichroická a absorpční spektrální studie interakcí DNA s 5,10,15,20-tetrakis(1-methylpyridinium-4-yl)porfyrinem (cs)
- Vibrational and Electronic Circular Dichroism and Absorption Spectral Study of the DNA - 5, 10, 15, 20-tetrakis(1-methylpyridinium-4-yl)porphyrin Interaction (en)
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| skos:prefLabel
| - Vibrational and Electronic Circular Dichroism and Absorption Spectral Study of the DNA - 5, 10, 15, 20-tetrakis(1-methylpyridinium-4-yl)porphyrin Interaction
- Vibračně a elektronově cirkulárně dichroická a absorpční spektrální studie interakcí DNA s 5,10,15,20-tetrakis(1-methylpyridinium-4-yl)porfyrinem (cs)
- Vibrational and Electronic Circular Dichroism and Absorption Spectral Study of the DNA - 5, 10, 15, 20-tetrakis(1-methylpyridinium-4-yl)porphyrin Interaction (en)
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| skos:notation
| - RIV/60461373:22340/05:00014756!RIV06-MSM-22340___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/60461373:22340/05:00014756
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - vibrational circular dichroism; electronic circular dichroism; IR absorption; DNA - TMPyP complexes (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Urbanová, Marie
- Volka, Karel
- Nový, Jakub
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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| is http://linked.open...avai/riv/vysledek
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