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| Description
| - The ultra-high performance liquid chromatography-quadrupole/time-of-flight mass spectrometry (UHPLC-QTOFMS) was applied to evaluate the potential of non-target metabolomic fingerprinting to distinguish Fusarium-infected and control barley samples. First, the sample extraction and UHPLC-QTOFMS instrumental conditions were optimized to obtain the broadest possible representation of polar/medium-polar compounds occurring in extracts obtained from barley grain samples. Next, the UHPLC-QTOFMS metabolomic fingerprints of extracts obtained from nine barley varieties were acquired under the conditions of electrospray ionization (ESI) in both positive and negative mode. Each variety of barley was tested in two variants: artificially infected by Fusarium culmorum at the beginning of heading and as control group (no infection). In addition, the dynamics of barley infection development was monitored using this approach. The experimental data were statistically evaluated by principal component analysis, hierarchical clustering analysis, and partial least squares discriminant analysis. Differentiation of barley in response to F. culmorum infection was feasible using this metabolomics-based method. Analysis in ESI(+) provided higher number of molecular features as compared to that performed under ESI(-) setting. However, the analysis in ESI(-) permitted the detection of deoxynivalenol and deoxynivalenol-3-glucoside considered as resistance-indicator metabolites in barley.
- The ultra-high performance liquid chromatography-quadrupole/time-of-flight mass spectrometry (UHPLC-QTOFMS) was applied to evaluate the potential of non-target metabolomic fingerprinting to distinguish Fusarium-infected and control barley samples. First, the sample extraction and UHPLC-QTOFMS instrumental conditions were optimized to obtain the broadest possible representation of polar/medium-polar compounds occurring in extracts obtained from barley grain samples. Next, the UHPLC-QTOFMS metabolomic fingerprints of extracts obtained from nine barley varieties were acquired under the conditions of electrospray ionization (ESI) in both positive and negative mode. Each variety of barley was tested in two variants: artificially infected by Fusarium culmorum at the beginning of heading and as control group (no infection). In addition, the dynamics of barley infection development was monitored using this approach. The experimental data were statistically evaluated by principal component analysis, hierarchical clustering analysis, and partial least squares discriminant analysis. Differentiation of barley in response to F. culmorum infection was feasible using this metabolomics-based method. Analysis in ESI(+) provided higher number of molecular features as compared to that performed under ESI(-) setting. However, the analysis in ESI(-) permitted the detection of deoxynivalenol and deoxynivalenol-3-glucoside considered as resistance-indicator metabolites in barley. (en)
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| Title
| - Rapid LC-MS-based metabolomics method to study the Fusarium infection of barley
- Rapid LC-MS-based metabolomics method to study the Fusarium infection of barley (en)
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| skos:prefLabel
| - Rapid LC-MS-based metabolomics method to study the Fusarium infection of barley
- Rapid LC-MS-based metabolomics method to study the Fusarium infection of barley (en)
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| skos:notation
| - RIV/60461373:22330/14:43898073!RIV15-MZE-22330___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/60461373:22330/14:43898073
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - mycotoxins; metabolomics; liquid chromatography-mass spectrometry (LC-MS); Fusarium head blight; Barley (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Journal of Separation Science
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Hajšlová, Jana
- Ovesná, Jaroslava
- Václavík, Lukáš
- Václavíková, Marta
- Čajka, Tomáš
- Džuman, Zbyněk
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| http://linked.open...ain/vavai/riv/wos
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| issn
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| number of pages
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| http://bibframe.org/vocab/doi
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| http://localhost/t...ganizacniJednotka
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