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  • Simultaneous saccharification and fermentation (SSF) of lignocellulosic substrate is limited by low glucose availability in the early stages of SSF, different temperature optima for enzymatic hydrolysis and fermentation, and low ethanol productivity. These were eliminated in this work by carrying out the pre-saccharification step at the optimum temperature for cellulolytic enzymes. When inoculation was delayed for 12 h (dSSF process), ethanol productivity was enhanced and the process was shortened by 60% compared to SSF, while the final ethanol concentration remained the same. Increased loading of acid-pretreated wheat straw in batch mode did not improve the ethanol concentration; although preconcentration of substrate from 36% to 66% of wet solid enabled a 48% increase in glucose concentration it negatively influenced fermentation. The fed-batch dSSF process, with gradual feeding of pre-hydrolysed medium, carried out in two bioreactors, enabled us to increase the final ethanol concentration and to eliminate problems associated with high concentrations of inhibitors in batch mode and high viscosity of the medium.
  • Simultaneous saccharification and fermentation (SSF) of lignocellulosic substrate is limited by low glucose availability in the early stages of SSF, different temperature optima for enzymatic hydrolysis and fermentation, and low ethanol productivity. These were eliminated in this work by carrying out the pre-saccharification step at the optimum temperature for cellulolytic enzymes. When inoculation was delayed for 12 h (dSSF process), ethanol productivity was enhanced and the process was shortened by 60% compared to SSF, while the final ethanol concentration remained the same. Increased loading of acid-pretreated wheat straw in batch mode did not improve the ethanol concentration; although preconcentration of substrate from 36% to 66% of wet solid enabled a 48% increase in glucose concentration it negatively influenced fermentation. The fed-batch dSSF process, with gradual feeding of pre-hydrolysed medium, carried out in two bioreactors, enabled us to increase the final ethanol concentration and to eliminate problems associated with high concentrations of inhibitors in batch mode and high viscosity of the medium. (en)
Title
  • High solid fed-batch SSF with delayed inoculation for improved production of bioethanol from wheat straw
  • High solid fed-batch SSF with delayed inoculation for improved production of bioethanol from wheat straw (en)
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  • High solid fed-batch SSF with delayed inoculation for improved production of bioethanol from wheat straw
  • High solid fed-batch SSF with delayed inoculation for improved production of bioethanol from wheat straw (en)
skos:notation
  • RIV/60461373:22330/14:43896443!RIV15-MSM-22330___
http://linked.open...avai/riv/aktivita
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  • P(ME10146)
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  • únor
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  • 19095
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  • RIV/60461373:22330/14:43896443
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  • high solid; fed-batch; saccharification; wheat straw; Bioethanol (en)
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  • GB - Spojené království Velké Británie a Severního Irska
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  • [6ED5FF37CF53]
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  • Fuel
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  • 122
http://linked.open...iv/tvurceVysledku
  • Melzoch, Karel
  • Patáková, Petra
  • Paulová, Leona
  • Rychtera, Mojmír
http://linked.open...ain/vavai/riv/wos
  • 000331544100034
issn
  • 0016-2361
number of pages
http://bibframe.org/vocab/doi
  • 10.1016/j.fuel.2014.01.020
http://localhost/t...ganizacniJednotka
  • 22330
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