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| rdf:type
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| rdfs:seeAlso
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| Description
| - As most cultivars of flax and linseed (Linum usitatissimum L.) are capable of accumulating cadmium (Cd), they are suitable candidates for phytoextraction of the metal from contaminated soils. In an attempt to enhance the phytoextraction capacity of L. usitatissimum through overproduction of an efficient heterologous Cd-binding peptide, we engineered linseed breeding line AGT 917 to constitutively express genetic fusion of alpha-domain of mammalian metallothionein 1a (alpha MT1a) and beta-glucuronidase gus gene under the control of CaMV 35S promoter. An improved transformation protocol was developed, which involved co-cultivation of AGT 917 hypocotyl segments with partially removed epidermis with Agrobacterium suspension for 10 min in the presence of 200 mg l(-1) cellulase. The enzyme treatment increased the transformation efficiency (TE) 1.6-fold as compared to agroinfection without cellulase. Less pronounced impact on TE exerted 100 mg l(-1) acetosyringone, increasing TE 1.3-fold. When tested in soils amended with Cd at 20 and 360 mg kg(-1), the mature alpha MT1a::gus plants accumulated more Cd than parental AGT 917: the stem Cd concentrations in the best performing alpha MT1/2 line were 3.3- and 1.9-fold higher, respectively. Moreover, hypocotyl explants of alpha MT1/2 line showed 1.7-fold higher biomass than those of AGT 917 on media containing 15 mg Cd l(-1), indicating that alpha MT1a::gus did confer higher Cd tolerance to engineered plant. Overproduction of metal-binding peptides thus appears to be a viable strategy for the production of L. usitatissimum with improved phytoremediation capacity.
- As most cultivars of flax and linseed (Linum usitatissimum L.) are capable of accumulating cadmium (Cd), they are suitable candidates for phytoextraction of the metal from contaminated soils. In an attempt to enhance the phytoextraction capacity of L. usitatissimum through overproduction of an efficient heterologous Cd-binding peptide, we engineered linseed breeding line AGT 917 to constitutively express genetic fusion of alpha-domain of mammalian metallothionein 1a (alpha MT1a) and beta-glucuronidase gus gene under the control of CaMV 35S promoter. An improved transformation protocol was developed, which involved co-cultivation of AGT 917 hypocotyl segments with partially removed epidermis with Agrobacterium suspension for 10 min in the presence of 200 mg l(-1) cellulase. The enzyme treatment increased the transformation efficiency (TE) 1.6-fold as compared to agroinfection without cellulase. Less pronounced impact on TE exerted 100 mg l(-1) acetosyringone, increasing TE 1.3-fold. When tested in soils amended with Cd at 20 and 360 mg kg(-1), the mature alpha MT1a::gus plants accumulated more Cd than parental AGT 917: the stem Cd concentrations in the best performing alpha MT1/2 line were 3.3- and 1.9-fold higher, respectively. Moreover, hypocotyl explants of alpha MT1/2 line showed 1.7-fold higher biomass than those of AGT 917 on media containing 15 mg Cd l(-1), indicating that alpha MT1a::gus did confer higher Cd tolerance to engineered plant. Overproduction of metal-binding peptides thus appears to be a viable strategy for the production of L. usitatissimum with improved phytoremediation capacity. (en)
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| Title
| - Enhanced accumulation of cadmium in Linum usitatissimum L. plants due to overproduction of metallothionein alpha-domain as a fusion to beta-glucuronidase protein
- Enhanced accumulation of cadmium in Linum usitatissimum L. plants due to overproduction of metallothionein alpha-domain as a fusion to beta-glucuronidase protein (en)
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| skos:prefLabel
| - Enhanced accumulation of cadmium in Linum usitatissimum L. plants due to overproduction of metallothionein alpha-domain as a fusion to beta-glucuronidase protein
- Enhanced accumulation of cadmium in Linum usitatissimum L. plants due to overproduction of metallothionein alpha-domain as a fusion to beta-glucuronidase protein (en)
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| skos:notation
| - RIV/60461373:22330/13:43895482!RIV14-MSM-22330___
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| http://linked.open...avai/predkladatel
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/60461373:22330/13:43895482
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Phytoextraction; Metallothionein; Linseed; Flax; Cadmium; Linum usitatissimum (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Plant Cell Tissue and Organ Culture
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Griga, Miroslav
- Horáček, Jiří
- Kotrba, Pavel
- Smýkalová, Iva
- Smýkal, Petr
- Vrbová, Miroslava
- Švábová, Lenka
- Větrovcová, Martina
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| http://linked.open...ain/vavai/riv/wos
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| issn
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| number of pages
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| http://bibframe.org/vocab/doi
| - 10.1007/s11240-012-0239-1
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| http://localhost/t...ganizacniJednotka
| |
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