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| Description
| - Strain of Actinobacillus succinogenes was selected for studies and optimization of succinic acid production. Anaerobic cultivations of many other producers of SA were carried out in complex glucose media with continuous supply of carbon dioxide (e.g. bacterial strain Basfia succiniciproducens). Laboratory results gave evidence about further steps of the research to obtain higher product concentrations, higher yields and productivities. New strains from various sources have been looked for. A great effort has been given to methods of bacteria conservation and propagation. Another task of the research was directed to ABE fermentation (aceton-butanol-ethanol process) using separately two strains of genus Clostridium which are not commonly used for similar type of studies. Solvent producing strain Clostridium pasteurianum was chosen as the most robust one for the improvement. For this glucose utilizing strain, a complete set of laboratory data including batch, fed-batch and continuous fermentations with suspended and immobilized cells have been documented. For C.beijerinckii utilizing sugar beet juice, it was demonstrated that the process can be transferred to a larger scale. It seems of interest to further investigate glycerol fermentation by C. pasteurianum because the yield is higher than in sugar fermentation and the process is not probably coupled with sporulation. Preliminary steps for genome sequenation of Clostridium pasteurianum and genome analysis were done with the aim of increasing and stabilizing butanol production. Development of continuous fermentation in packed-bed/mixed bioreactor using glycerol as a substrate and immobilized/free cells of the promising strain, Clostridium pasteurianum was performed. The main objective of the research is to prepare technology for both products in laboratory suitable for further technology transfer into pilot scale.
- Strain of Actinobacillus succinogenes was selected for studies and optimization of succinic acid production. Anaerobic cultivations of many other producers of SA were carried out in complex glucose media with continuous supply of carbon dioxide (e.g. bacterial strain Basfia succiniciproducens). Laboratory results gave evidence about further steps of the research to obtain higher product concentrations, higher yields and productivities. New strains from various sources have been looked for. A great effort has been given to methods of bacteria conservation and propagation. Another task of the research was directed to ABE fermentation (aceton-butanol-ethanol process) using separately two strains of genus Clostridium which are not commonly used for similar type of studies. Solvent producing strain Clostridium pasteurianum was chosen as the most robust one for the improvement. For this glucose utilizing strain, a complete set of laboratory data including batch, fed-batch and continuous fermentations with suspended and immobilized cells have been documented. For C.beijerinckii utilizing sugar beet juice, it was demonstrated that the process can be transferred to a larger scale. It seems of interest to further investigate glycerol fermentation by C. pasteurianum because the yield is higher than in sugar fermentation and the process is not probably coupled with sporulation. Preliminary steps for genome sequenation of Clostridium pasteurianum and genome analysis were done with the aim of increasing and stabilizing butanol production. Development of continuous fermentation in packed-bed/mixed bioreactor using glycerol as a substrate and immobilized/free cells of the promising strain, Clostridium pasteurianum was performed. The main objective of the research is to prepare technology for both products in laboratory suitable for further technology transfer into pilot scale. (cs)
- Kmen Actinobacillus succinogenes byl vybrán pro stadium a optimalizaci produkce jantarové kyseliny. Byly realizovány anaerobní kultivace mnoha dalších producentů jantarové kyseliny s otestováním komplexních glukosových medií s nepřetržitým přívodem oxidu uhličitého (např. bakteriální kmen Basfia succiniciproducens). Laboratorní výsledky sloužily k vytvoření strategie pro další výzkum s cílem zvýšit koncentraci produktu v médiu, zvýšit výtěžnosti a produktivitu jantarové kyseliny. Hledány byly produkční kmeny různého původu. Velký podíl prací byl věnován stadiu způsobů konzervace a propagace kmenů. Další výzkumný úkol byl orientován na ABE fermentaci (aceton-butanol-ethanolový proces) s využitím dvou kmenů rodu Clostridium, které nejsou běžně používány při podobných studiích. Byl vybrán solventogenní kmen Clostridium pasteurianum, který se vyznačuje robustností a tím i předpokladem pro zlepšení vlastností. Pro tento mikroorganismus byla experimentálně získán soubor dat ze vsádkových, přítokovaných a i kontinuálních fermentací a to jak s buňkami volnými, tak i imobilizovanými. Pro C.beijerinckii využívající cukernou šťávu se ukázalo, že tento proces může být převeden do většího měřítka. Pro další výzkum je zajímavé a povzbudivé, že bakterie C. pasteurianum dává na glycerolu vyšší výtěžky než na cukerných substrátech a že proces není pravděpodobně spojen se sporulací. Byly provedeny předběžné zkoušky a přípravy k sekvenování genomu bakterie Clostridium pasteurianum a pro genovou analýzu s cílem zvýšit a stabilizovat v budoucnosti vyšší produkci butanolu. Byly provedeny zkoušky s kontinuální fermentací v náplňovém/míchaném reaktoru s volnými a imobilizovanými buňkami nadějného kmene Clostridium pasteurianum. (en)
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| Title
| - Vývoj technologie pro fermentační produkci butanolu a jantarové kyseliny
- Vývoj technologie pro fermentační produkci butanolu a jantarové kyseliny (cs)
- Technology development for butanol and succinic acid fermentation processes (en)
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| skos:prefLabel
| - Vývoj technologie pro fermentační produkci butanolu a jantarové kyseliny
- Vývoj technologie pro fermentační produkci butanolu a jantarové kyseliny (cs)
- Technology development for butanol and succinic acid fermentation processes (en)
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| skos:notation
| - RIV/60461373:22330/12:43896901!RIV15-MSM-22330___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/60461373:22330/12:43896901
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - processes; fermentation; acid; succinic; and; butanol; for; development; Technology (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...telVyzkumneZpravy
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...iv/tvurceVysledku
| - Melzoch, Karel
- Patáková, Petra
- Paulová, Leona
- Rychtera, Mojmír
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| http://linked.open...rzeVyzkumneZpravy
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| number of pages
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| http://localhost/t...ganizacniJednotka
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