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| Description
| - An increasing incidence of T-2 and HT-2 toxins (T-2 and HT-2) representing group A trichothecenes has been observed in Fusarium infected small grain cereals in the last decade. Quite recently, the existence of glucosides of T-2 and HT-2 has also been proved next to the common parent forms. There is a strong desire to deepen the knowledge of the occurrence ot these conjugates and the possibilities of their analytical determination. A new analytical procedure has been developed for monitoring T-2/HT-2 conjugates in cereal samples. Four different sample preparation methods based on crude acetonitrile:water extraction, QuEChERS, solid phase extraction ind immunoaffinity clean-up, were tested. The latter approach employing dedicated immunoaffinity cross-reactive cartridges was shown to be the best option for selective isolation and pre-concentration of the target conjugated analytes. The samples obtained in this way were examined by ultra-high performance liquid chromatography hyphenated to high resolution tandem mass spectrometry that enabled the confirmation of the presence of conjugated T-2 and HT-2. In addition to mono-glycosylated forms of T-2 and HT-2 detected in naturally contaminated barley, wheat and oats, we have also documented for the first time the existence of diglucosides of HT-2 in barley.
- An increasing incidence of T-2 and HT-2 toxins (T-2 and HT-2) representing group A trichothecenes has been observed in Fusarium infected small grain cereals in the last decade. Quite recently, the existence of glucosides of T-2 and HT-2 has also been proved next to the common parent forms. There is a strong desire to deepen the knowledge of the occurrence ot these conjugates and the possibilities of their analytical determination. A new analytical procedure has been developed for monitoring T-2/HT-2 conjugates in cereal samples. Four different sample preparation methods based on crude acetonitrile:water extraction, QuEChERS, solid phase extraction ind immunoaffinity clean-up, were tested. The latter approach employing dedicated immunoaffinity cross-reactive cartridges was shown to be the best option for selective isolation and pre-concentration of the target conjugated analytes. The samples obtained in this way were examined by ultra-high performance liquid chromatography hyphenated to high resolution tandem mass spectrometry that enabled the confirmation of the presence of conjugated T-2 and HT-2. In addition to mono-glycosylated forms of T-2 and HT-2 detected in naturally contaminated barley, wheat and oats, we have also documented for the first time the existence of diglucosides of HT-2 in barley. (en)
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| Title
| - Occurrence of mono- and di-glycosylated conjugates of T-2 and HT-2 toxins in naturally contaminated cereals
- Occurrence of mono- and di-glycosylated conjugates of T-2 and HT-2 toxins in naturally contaminated cereals (en)
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| skos:prefLabel
| - Occurrence of mono- and di-glycosylated conjugates of T-2 and HT-2 toxins in naturally contaminated cereals
- Occurrence of mono- and di-glycosylated conjugates of T-2 and HT-2 toxins in naturally contaminated cereals (en)
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| skos:notation
| - RIV/60461373:22330/12:43894815!RIV13-MZE-22330___
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| http://linked.open...avai/predkladatel
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/60461373:22330/12:43894815
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - UPLC-QqTOF MS; high resolution mass spectrometry; T-2 toxin; HT-2 toxin; masked mycotoxins (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Hajšlová, Jana
- Václavíková, Marta
- Zachariášová, Milena
- Džuman, Zbyněk
- Vepříková, Zdenka
- Lacina, Ondřej
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| http://linked.open...ain/vavai/riv/wos
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| issn
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| number of pages
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| http://bibframe.org/vocab/doi
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| http://localhost/t...ganizacniJednotka
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| is http://linked.open...avai/riv/vysledek
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