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  • Bee pollen is a perspective product of beekeeping successfully used as food supplements. Bee pollen and honeycomb pollen (perga) are sources of many biologically active and nutritive compounds. Discrimination of these materials according to their botanic origin is necessary to evaluate their quality and possible applications. Diffuse reflectance VIS spectroscopy was applied to analysis of 7 samples of monofloral bee pollen and 2 samples of honeycomb pollen of unknown origin. The spectra were used for calculation of CIE Lab colour parameters. Classification of the samples was based on multivariate analysis of the spectra. CIE Lab diagram, HCA dendrogram and PCA score plot led to separation of the samples according to their difference in pigmentation. FTIR spectra of the samples illustrate differences in composition and heterogeneity of some bee pollens. Microscopic image analysis of pollen granules demonstrated evident variability in size, morphology and colour depending on the botanical origin. Differently coloured granules or pollen grains on their surface may be explained by some heterogeneity in botanical composition. Spectroscopic (Vis, FTIR) and microscopic methods confirmed to be effective screening method for pollen analysis.
  • Bee pollen is a perspective product of beekeeping successfully used as food supplements. Bee pollen and honeycomb pollen (perga) are sources of many biologically active and nutritive compounds. Discrimination of these materials according to their botanic origin is necessary to evaluate their quality and possible applications. Diffuse reflectance VIS spectroscopy was applied to analysis of 7 samples of monofloral bee pollen and 2 samples of honeycomb pollen of unknown origin. The spectra were used for calculation of CIE Lab colour parameters. Classification of the samples was based on multivariate analysis of the spectra. CIE Lab diagram, HCA dendrogram and PCA score plot led to separation of the samples according to their difference in pigmentation. FTIR spectra of the samples illustrate differences in composition and heterogeneity of some bee pollens. Microscopic image analysis of pollen granules demonstrated evident variability in size, morphology and colour depending on the botanical origin. Differently coloured granules or pollen grains on their surface may be explained by some heterogeneity in botanical composition. Spectroscopic (Vis, FTIR) and microscopic methods confirmed to be effective screening method for pollen analysis. (en)
Title
  • Spectroscopic discrimination of bee pollen and honeycomb pollen samples
  • Spectroscopic discrimination of bee pollen and honeycomb pollen samples (en)
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  • Spectroscopic discrimination of bee pollen and honeycomb pollen samples
  • Spectroscopic discrimination of bee pollen and honeycomb pollen samples (en)
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  • RIV/60461373:22330/12:43894681!RIV13-MSM-22330___
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  • Z(MSM6046137305)
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  • 170372
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  • RIV/60461373:22330/12:43894681
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  • bee pollen, honeycomb pollen, spectroscopic methods, discrimination (en)
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  • [4F39206C47ED]
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  • Brno
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  • Brno
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  • CECE 2012 9th International interdisciplinary meeting on bioanalysis
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  • Synytsya, Andriy
  • Bleha, Roman
  • Brindza, Ján
  • Kucelová, Lucie
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http://linked.open.../riv/zahajeniAkce
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  • Ústav Analytické chemie AV ČR, v.v.i.
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  • 978-80-904959-1-3
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  • 22330
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