About: SUMO-2/3 conjugates accumulating under heat shock or MG132 treatment result largely from new protein synthesis

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About: SUMO-2/3 conjugates accumulating under heat shock or MG132 treatment result largely from new protein synthesis Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Small ubiquitin-related modifiers 1, 2 and 3 (SUMO-1, -2, -3), members of the ubiquitin-like protein family, can be conjugated to various cellular proteins. Conjugates of SUMO-2 and SUMO-3 (SUMO-2/3) accumulate in cells exposed to various stress stimuli or to MG132 treatment. Although the proteins modified by SUMO-2/3 during heat shock or under MG132 treatment have been identified, the significance of this modification remains unclear. Our data show that the inhibition of translation by puromycin or cycloheximide blocks both the heat shock and MG132 induced accumulation of SUMO-2/3 conjugates in HEK 293T and U2OS cells. However, the heat shock induced accumulation of SUMO-2/3 conjugates was restored by proteasome inhibition, which suggests that the inhibition of translation did not abolish SUMOylation itself. Furthermore, we show that some of the proteins truncated due to the treatment by low concentration of puromycin are SUMOylated in HEK 293T cells. We suggest that the SUMO-2/3 conjugates accumulating under the heat shock or MG132 treatment result largely from new protein synthesis and that portion of them is incorrectly folded. Small ubiquitin-related modifiers 1, 2 and 3 (SUMO-1, -2, -3), members of the ubiquitin-like protein family, can be conjugated to various cellular proteins. Conjugates of SUMO-2 and SUMO-3 (SUMO-2/3) accumulate in cells exposed to various stress stimuli or to MG132 treatment. Although the proteins modified by SUMO-2/3 during heat shock or under MG132 treatment have been identified, the significance of this modification remains unclear. Our data show that the inhibition of translation by puromycin or cycloheximide blocks both the heat shock and MG132 induced accumulation of SUMO-2/3 conjugates in HEK 293T and U2OS cells. However, the heat shock induced accumulation of SUMO-2/3 conjugates was restored by proteasome inhibition, which suggests that the inhibition of translation did not abolish SUMOylation itself. Furthermore, we show that some of the proteins truncated due to the treatment by low concentration of puromycin are SUMOylated in HEK 293T cells. We suggest that the SUMO-2/3 conjugates accumulating under the heat shock or MG132 treatment result largely from new protein synthesis and that portion of them is incorrectly folded. (en)
Title	SUMO-2/3 conjugates accumulating under heat shock or MG132 treatment result largely from new protein synthesis SUMO-2/3 conjugates accumulating under heat shock or MG132 treatment result largely from new protein synthesis (en)
skos:prefLabel	SUMO-2/3 conjugates accumulating under heat shock or MG132 treatment result largely from new protein synthesis SUMO-2/3 conjugates accumulating under heat shock or MG132 treatment result largely from new protein synthesis (en)
skos:notation	RIV/60461373:22330/12:43894098!RIV13-MSM-22330___
http://linked.open...avai/predkladatel	Fakulta potravinářské a biochemické technologie
http://linked.open...avai/riv/aktivita	S Z
http://linked.open...avai/riv/aktivity	S, Z(MSM6046137305)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Lipov, Jan Častorálová, Markéta Březinová, Dagmar Švéda, Martin Knejzlík, Zdeněk Ruml, Tomáš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta potravinářské a biochemické technologie
http://linked.open...dnocenehoVysledku	172443
http://linked.open...ai/riv/idVysledku	RIV/60461373:22330/12:43894098
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	17-AAG; MG132; Heat shock; Protein synthesis; SUMOylation; SUMO (en)
http://linked.open.../riv/klicoveSlovo	17-AAG Heat shock MG132 Protein synthesis SUMO SUMOylation
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[97781DABCEB4]
http://linked.open...i/riv/nazevZdroje	Biochimica Et Biophysica Acta-Molecular Cell Research
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	1823
http://linked.open...iv/tvurceVysledku	Lipov, Jan Ruml, Tomáš Častorálová, Markéta Knejzlík, Zdeněk Březinová, Dagmar Švéda, Martin
http://linked.open...ain/vavai/riv/wos	000302842500012
http://linked.open...n/vavai/riv/zamer	Teoretické základy potravinářských a biochemických technologií
issn	0167-4889
number of pages	9 (xsd:int)
http://localhost/t...ganizacniJednotka	22330
is http://linked.open...avai/riv/vysledek of	SUMO-2/3 conjugates accumulating under heat shock or MG132 treatment result largely from new protein synthesis

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