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  • Reductive dehalogenation by anaerobic bacteria is a promising method of detoxifying polychlorinated biphenyls (PCBs) in aquatic sediments. PCB-contaminated sediment from Strazsky Sewer river (Slovakia) was used as an inoculum to develop stable sediment-free enrichments with dechlorination activity directed against the commercial PCB mixture Aroclor 1260. RESULTS: Characterization of the PCBs at the site revealed that 45 mol% of the PCBs were dichlorobiphenyls and 62% of the chlorines were in the ortho-position. Both findings are characteristic of extensive dechlorination in situ. Sediment-free enrichments with stable dechlorinating activity against Aroclor 1260 were developed by a series of transfers. These enrichments targeted hexa- through nonachlorobiphenyls and dechlorinated them to tri- through hexachlorobiphenyls by removal of flanked meta- and para- chlorines when pyruvate or lactate was added as a carbon source. The known haloprimers: 4,4'-dibromobiphenyl and 2,6-dibromobiphenyl did not influence the rate or pattern of dechlorination. The chlorophenyl rings targeted were 2,3,4,5,6- (23456-), 2346-, 2345-, 234- and 245-. CONCLUSION: The PCB dechlorination carried out by these enrichments is novel and does not correspond to any of the previously identified microbial PCB dechlorination processes or their combination. Natural attenuation processes may be under way at the Strazsky Sewer river site.
  • Reductive dehalogenation by anaerobic bacteria is a promising method of detoxifying polychlorinated biphenyls (PCBs) in aquatic sediments. PCB-contaminated sediment from Strazsky Sewer river (Slovakia) was used as an inoculum to develop stable sediment-free enrichments with dechlorination activity directed against the commercial PCB mixture Aroclor 1260. RESULTS: Characterization of the PCBs at the site revealed that 45 mol% of the PCBs were dichlorobiphenyls and 62% of the chlorines were in the ortho-position. Both findings are characteristic of extensive dechlorination in situ. Sediment-free enrichments with stable dechlorinating activity against Aroclor 1260 were developed by a series of transfers. These enrichments targeted hexa- through nonachlorobiphenyls and dechlorinated them to tri- through hexachlorobiphenyls by removal of flanked meta- and para- chlorines when pyruvate or lactate was added as a carbon source. The known haloprimers: 4,4'-dibromobiphenyl and 2,6-dibromobiphenyl did not influence the rate or pattern of dechlorination. The chlorophenyl rings targeted were 2,3,4,5,6- (23456-), 2346-, 2345-, 234- and 245-. CONCLUSION: The PCB dechlorination carried out by these enrichments is novel and does not correspond to any of the previously identified microbial PCB dechlorination processes or their combination. Natural attenuation processes may be under way at the Strazsky Sewer river site. (en)
Title
  • Sediment-free anaerobic microbial enrichments with novel dechlorinating activity against highly chlorinated commercial PCBs
  • Sediment-free anaerobic microbial enrichments with novel dechlorinating activity against highly chlorinated commercial PCBs (en)
skos:prefLabel
  • Sediment-free anaerobic microbial enrichments with novel dechlorinating activity against highly chlorinated commercial PCBs
  • Sediment-free anaerobic microbial enrichments with novel dechlorinating activity against highly chlorinated commercial PCBs (en)
skos:notation
  • RIV/60461373:22330/12:43893576!RIV13-GA0-22330___
http://linked.open...avai/predkladatel
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(1M06011), P(GA525/09/1058)
http://linked.open...iv/cisloPeriodika
  • 9
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 167199
http://linked.open...ai/riv/idVysledku
  • RIV/60461373:22330/12:43893576
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • PCBs; Aroclor 1260; Dechlorination (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV
  • [2156CE30A7C2]
http://linked.open...i/riv/nazevZdroje
  • Journal of Chemical Technology and biotechnology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 87
http://linked.open...iv/tvurceVysledku
  • Demnerová, Kateřina
  • Dudková, Vlasta
  • Dedard, D.L
http://linked.open...ain/vavai/riv/wos
  • 000307558500006
issn
  • 1097-4660
number of pages
http://localhost/t...ganizacniJednotka
  • 22330
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