About: Disulfide bond decay during matrix-assisted laser desorption/ionization time-of-flight mass spectrometry experiments

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About: Disulfide bond decay during matrix-assisted laser desorption/ionization time-of-flight mass spectrometry experiments Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In our laboratory, we have been studying the reductive processes that occur during matrix-assisted laser desorption/ionization (MALDI) experiments. Recently, we have finished an analysis of the DHB matrix effect on the azo group in cyclic peptides. However, deep understanding of disulfide bond behaviour during a mass spectrometry (MS) experiment is much more important in proteomics as its reduction can cause serious errors in protein spectra interpretation. Therefore, we have focused on Intra- and intermolecular disulfide bonds as well as disulfide bonds connecting cysteine and 2-thio-5-nitrobenzoic acid (TNB, Ellman's reagent modification) in model peptides during MALDI MS measurements. While the reduction was not observed for Intra- and intermolecular cysteine-cysteine disulfide bonds, the disulfide connection between cysteine and TNB was always affected. It was proved that TNB and Ellman's reagent can act as a matrix itself. The results obtained enabled us to propose a reaction mechanism model which is able to describe the phenomena observed during the desorption/ionization process of disulfide-containing molecules. In our laboratory, we have been studying the reductive processes that occur during matrix-assisted laser desorption/ionization (MALDI) experiments. Recently, we have finished an analysis of the DHB matrix effect on the azo group in cyclic peptides. However, deep understanding of disulfide bond behaviour during a mass spectrometry (MS) experiment is much more important in proteomics as its reduction can cause serious errors in protein spectra interpretation. Therefore, we have focused on Intra- and intermolecular disulfide bonds as well as disulfide bonds connecting cysteine and 2-thio-5-nitrobenzoic acid (TNB, Ellman's reagent modification) in model peptides during MALDI MS measurements. While the reduction was not observed for Intra- and intermolecular cysteine-cysteine disulfide bonds, the disulfide connection between cysteine and TNB was always affected. It was proved that TNB and Ellman's reagent can act as a matrix itself. The results obtained enabled us to propose a reaction mechanism model which is able to describe the phenomena observed during the desorption/ionization process of disulfide-containing molecules. (en)
Title	Disulfide bond decay during matrix-assisted laser desorption/ionization time-of-flight mass spectrometry experiments Disulfide bond decay during matrix-assisted laser desorption/ionization time-of-flight mass spectrometry experiments (en)
skos:prefLabel	Disulfide bond decay during matrix-assisted laser desorption/ionization time-of-flight mass spectrometry experiments Disulfide bond decay during matrix-assisted laser desorption/ionization time-of-flight mass spectrometry experiments (en)
skos:notation	RIV/60461373:22330/11:43891997!RIV12-MSM-22330___
http://linked.open...avai/predkladatel	Fakulta potravinářské a biochemické technologie
http://linked.open...avai/riv/aktivita	S Z
http://linked.open...avai/riv/aktivity	S, Z(MSM6046137305)
http://linked.open...iv/cisloPeriodika	17
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Krásný, Lukáš Hynek, Radovan Kodíček, Milan
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta potravinářské a biochemické technologie
http://linked.open...dnocenehoVysledku	194974
http://linked.open...ai/riv/idVysledku	RIV/60461373:22330/11:43891997
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Acid; Reduction; Peptides; Sulfhydryl-groups (en)
http://linked.open.../riv/klicoveSlovo	Acid Peptides Reduction Sulfhydryl-groups
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[FDD07C962E1F]
http://linked.open...i/riv/nazevZdroje	Rapid Communications in Mass Spectrometry
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	25
http://linked.open...iv/tvurceVysledku	Hynek, Radovan Kodíček, Milan Krásný, Lukáš
http://linked.open...ain/vavai/riv/wos	000294267200014
http://linked.open...n/vavai/riv/zamer	Teoretické základy potravinářských a biochemických technologií
issn	0951-4198
number of pages	7 (xsd:int)
http://bibframe.org/vocab/doi	10.1002/rcm.5140
http://localhost/t...ganizacniJednotka	22330
is http://linked.open...avai/riv/vysledek of	Disulfide bond decay during matrix-assisted laser desorption/ionization time-of-flight mass spectrometry experiments Disulfide bond decay during matrix-assisted laser desorption/ionization time-of-flight mass spectrometry experiments

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