About: Analysis of isoflavonoids in Ficus carica by HPLC-MS and a comparison of extract Cleanup by Immunoaffinity Chromatography (IAC) and Solid Phase Extraction (SPE)

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About: Analysis of isoflavonoids in Ficus carica by HPLC-MS and a comparison of extract Cleanup by Immunoaffinity Chromatography (IAC) and Solid Phase Extraction (SPE) Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Isoflavonoids are plant phenolics known for their multiple pharmacological activities such as estrogenic, antibacterial, antioxidative etc. While legumes are their main source, the presence of isoflavonoids has been reported in about fifty other families. In non-legumes, isoflavonoids may be present in low concentrations, which complicates their detection and identification in complex biological matrixes. Two methods of sample clean-up prior to HPLC-ESI-MS, i.e. SPE (Solid Phase Extraction) and IAC (Immunoaffinity Chromatography), were compared. Conventional C18 reversed-phase sorbent cartridges (Waters Oasis, 30 ?m) were used for SPE. Immunosorbents for IAC were prepared by immobilisation of polyclonal rabbit antibodies against isoflavonoid-BSA (i.e. genistein, biochanin A and daidzein) conjugates on Affi-Gel 10 solid support (Bio-Rad Laboratories). A set of isoflavonoid standards and edible fig?s (Ficus carica) leaves and fruit ethanolic extracts were cleaned-up by two above mentioned methods and Isoflavonoids are plant phenolics known for their multiple pharmacological activities such as estrogenic, antibacterial, antioxidative etc. While legumes are their main source, the presence of isoflavonoids has been reported in about fifty other families. In non-legumes, isoflavonoids may be present in low concentrations, which complicates their detection and identification in complex biological matrixes. Two methods of sample clean-up prior to HPLC-ESI-MS, i.e. SPE (Solid Phase Extraction) and IAC (Immunoaffinity Chromatography), were compared. Conventional C18 reversed-phase sorbent cartridges (Waters Oasis, 30 ?m) were used for SPE. Immunosorbents for IAC were prepared by immobilisation of polyclonal rabbit antibodies against isoflavonoid-BSA (i.e. genistein, biochanin A and daidzein) conjugates on Affi-Gel 10 solid support (Bio-Rad Laboratories). A set of isoflavonoid standards and edible fig?s (Ficus carica) leaves and fruit ethanolic extracts were cleaned-up by two above mentioned methods and (en)
Title	Analysis of isoflavonoids in Ficus carica by HPLC-MS and a comparison of extract Cleanup by Immunoaffinity Chromatography (IAC) and Solid Phase Extraction (SPE) Analysis of isoflavonoids in Ficus carica by HPLC-MS and a comparison of extract Cleanup by Immunoaffinity Chromatography (IAC) and Solid Phase Extraction (SPE) (en)
skos:prefLabel	Analysis of isoflavonoids in Ficus carica by HPLC-MS and a comparison of extract Cleanup by Immunoaffinity Chromatography (IAC) and Solid Phase Extraction (SPE) Analysis of isoflavonoids in Ficus carica by HPLC-MS and a comparison of extract Cleanup by Immunoaffinity Chromatography (IAC) and Solid Phase Extraction (SPE) (en)
skos:notation	RIV/60461373:22330/10:00024198!RIV11-GA0-22330___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA525/09/0994), Z(MSM6046137305)
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Prokudina, Jelena Alexandrovna Lanková, Petra Lapčík, Oldřich Mikšátková, Petra
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta potravinářské a biochemické technologie
http://linked.open...dnocenehoVysledku	246417
http://linked.open...ai/riv/idVysledku	RIV/60461373:22330/10:00024198
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	IMMUNOASSAY; HPLC-MS; ISOFLAVONE; MORACEAE IMMUNOAFFINYTY EXTRACTION (en)
http://linked.open.../riv/klicoveSlovo	HPLC-MS ISOFLAVONE MORACEAE IMMUNOAFFINYTY EXTRACTION IMMUNOASSAY
http://linked.open...ontrolniKodProRIV	[F585D3E6C752]
http://linked.open...v/mistoKonaniAkce	Montpellier, Francie
http://linked.open...i/riv/mistoVydani	Montpellier
http://linked.open...i/riv/nazevZdroje	Polyphenols Communications 2010
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Chemotaxonomy of isoflavonoids
http://linked.open...UplatneniVysledku	2010
http://linked.open...iv/tvurceVysledku	Lapčík, Oldřich Lanková, Petra Prokudina, Jelena Alexandrovna Mikšátková, Petra
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2010-01-01 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Teoretické základy potravinářských a biochemických technologií
number of pages	2 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Groupe Polyphénols
https://schema.org/isbn	978-2-7380-1282-1
http://localhost/t...ganizacniJednotka	22330
is http://linked.open...avai/riv/vysledek of	Analysis of isoflavonoids in Ficus carica by HPLC-MS and a comparison of extract Cleanup by Immunoaffinity Chromatography (IAC) and Solid Phase Extraction (SPE)

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