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Description
| - Three bacteriocins were purified from lactic acid bacteria isolated from mayonnaise-based finished products and the raw materials used in the production of the finished products. All three bacteriocin-producing strains (EN3a, EN14a, and EN15b) were identified by sequencing of the 16S rDNA as Lactococcus lactis. The RP-HPLC fractions showed strong antimicrobial activity against Listeria monocytogenes. Mass spectrometry analysis of active fractions showed that all strains produced nisin A (3,353.7 Da). These results were confirmed by amplification of nisin structural gene by PCR, by lack of activity against a nisin-producing strain, and by total loss of activity after reaction of purified bacteriocins with glutathione. The strains Lactococcus lactis EN3a, EN14a, and EN15b are the first nisin-producing strains isolated from mayonnaise-based commercial products and from the raw materials used in their production. The ability of these strains to produce nisin could be useful to improve food quality of thes
- Three bacteriocins were purified from lactic acid bacteria isolated from mayonnaise-based finished products and the raw materials used in the production of the finished products. All three bacteriocin-producing strains (EN3a, EN14a, and EN15b) were identified by sequencing of the 16S rDNA as Lactococcus lactis. The RP-HPLC fractions showed strong antimicrobial activity against Listeria monocytogenes. Mass spectrometry analysis of active fractions showed that all strains produced nisin A (3,353.7 Da). These results were confirmed by amplification of nisin structural gene by PCR, by lack of activity against a nisin-producing strain, and by total loss of activity after reaction of purified bacteriocins with glutathione. The strains Lactococcus lactis EN3a, EN14a, and EN15b are the first nisin-producing strains isolated from mayonnaise-based commercial products and from the raw materials used in their production. The ability of these strains to produce nisin could be useful to improve food quality of thes (en)
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Title
| - Nisin-producing Lactococcus spp. from mayonnaise-based products and their raw materials
- Nisin-producing Lactococcus spp. from mayonnaise-based products and their raw materials (en)
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skos:prefLabel
| - Nisin-producing Lactococcus spp. from mayonnaise-based products and their raw materials
- Nisin-producing Lactococcus spp. from mayonnaise-based products and their raw materials (en)
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skos:notation
| - RIV/60461373:22330/10:00024158!RIV11-MSM-22330___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/60461373:22330/10:00024158
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - Bacteriocin; Nisin; Mayonnaise; Chili spice; Lactococcus lactis; Listeria monocytogenes (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - European Food Research and Technology
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Chumchalová, Jana
- Miller, Petr
- McMullen, Lynn
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http://linked.open...ain/vavai/riv/wos
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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http://localhost/t...ganizacniJednotka
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is http://linked.open...avai/riv/vysledek
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