About: Development of flow cytometry technique for detection of thinning of peptidoglycan layer as a result of solvent production by Clostridium pasteurianum

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About: Development of flow cytometry technique for detection of thinning of peptidoglycan layer as a result of solvent production by Clostridium pasteurianum Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Clostridium pasteurianum forms acetic and butyric acids in an initial growth phase. It is a typical feature of clostridial acetone-butanol fermentation where an initial accumulation of acids is followed by production of solvents: 1-butanol, acetone and ethanol. The initiation of the solvents production coupled with endospore formation leads to decrease of the cell wall thickness. The thinner cell wall is more resistant against solvents and dyes. These changes can be observed by the method originated in Gram staining. The cell wall of G+ bacteria allows the entry of hexidium iodide (HI) and rhodamine 123 (RH123). However, the outer membrane of G- bacteria does not allow the uptake and therefore G+ bacteria are stained with higher fluorescence intensity than G- bacteria. The ratio of fluorescence intensity to forward scatter (FSC) was determined to correspond to G+ bacteria when clostridia were producing less of solvents. The significant drop of the ratio of fluorescence intensity to FSC to the level co Clostridium pasteurianum forms acetic and butyric acids in an initial growth phase. It is a typical feature of clostridial acetone-butanol fermentation where an initial accumulation of acids is followed by production of solvents: 1-butanol, acetone and ethanol. The initiation of the solvents production coupled with endospore formation leads to decrease of the cell wall thickness. The thinner cell wall is more resistant against solvents and dyes. These changes can be observed by the method originated in Gram staining. The cell wall of G+ bacteria allows the entry of hexidium iodide (HI) and rhodamine 123 (RH123). However, the outer membrane of G- bacteria does not allow the uptake and therefore G+ bacteria are stained with higher fluorescence intensity than G- bacteria. The ratio of fluorescence intensity to forward scatter (FSC) was determined to correspond to G+ bacteria when clostridia were producing less of solvents. The significant drop of the ratio of fluorescence intensity to FSC to the level co (en)
Title	Development of flow cytometry technique for detection of thinning of peptidoglycan layer as a result of solvent production by Clostridium pasteurianum Development of flow cytometry technique for detection of thinning of peptidoglycan layer as a result of solvent production by Clostridium pasteurianum (en)
skos:prefLabel	Development of flow cytometry technique for detection of thinning of peptidoglycan layer as a result of solvent production by Clostridium pasteurianum Development of flow cytometry technique for detection of thinning of peptidoglycan layer as a result of solvent production by Clostridium pasteurianum (en)
skos:notation	RIV/60461373:22330/10:00023765!RIV11-MPO-22330___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(FR-TI1/218), P(QH81323), S, Z(MSM6046137305)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Melzoch, Karel Rychtera, Mojmír Patáková, Petra Fribert, Petr Linhová, Michaela Lipovský, Jakub Paulová, Leona
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta potravinářské a biochemické technologie
http://linked.open...dnocenehoVysledku	253945
http://linked.open...ai/riv/idVysledku	RIV/60461373:22330/10:00023765
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Flow cytometry; Gram staining; Clostridium pasteurianum; 1-butanol (en)
http://linked.open.../riv/klicoveSlovo	1-butanol Clostridium pasteurianum Flow cytometry Gram staining
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[EBCEB5D698A8]
http://linked.open...i/riv/nazevZdroje	Folia Microbiologica
http://linked.open...in/vavai/riv/obor	EI
http://linked.open...ichTvurcuVysledku	7 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	*THE DESIGN OF THE PRODUCTION OF BUTANOL WITH THE PILOT PLANT TEST VERIFICATION Biobutanol as a perspective renewable source of energy for transport
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	55
http://linked.open...iv/tvurceVysledku	Fribert, Petr Linhová, Michaela Lipovský, Jakub Melzoch, Karel Patáková, Petra Paulová, Leona Rychtera, Mojmír
http://linked.open...ain/vavai/riv/wos	000281277900009
http://linked.open...n/vavai/riv/zamer	Teoretické základy potravinářských a biochemických technologií
issn	0015-5632
number of pages	5 (xsd:int)
http://localhost/t...ganizacniJednotka	22330
is http://linked.open...avai/riv/vysledek of	Development of flow cytometry technique for detection of thinning of peptidoglycan layer as a result of solvent production by Clostridium pasteurianum

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