| Attributes | Values |
|---|
| rdf:type
| |
| Description
| - The growth kinetic, overproduction of excreted recombinant protein and cell physiology of Pichia pastoris Mut+ strain were studied on mixed substrate containing two carbon sources (glucose and methanol) in chemostat. Although glucose is considered as a repressive substrate for AOX promoter, recombinant trypsinogen was expressed when glucose was used in mixture with methanol in substrate limited culture. The specific methanol uptake rate was rising with increasing dilution rate reaching maximum 0.090.01 g.gCDW-1.h-1 at 0.15 h-1; both co-substrates were utilised simultaneously and fully. At dilution rates, exceeding 0.15 h-1 the specific methanol uptake rate was decreasing and a residual methanol was detected in the culture broth. On the other hand, the specific glucose uptake rate was increasing within the interval of measured dilution rates reaching maximum (0.240.01) g.gCDW-1.h-1 at 0.2 h-1. In case of continuous cultures performed at higher dilution rates the yeasts preferred the higher carbon sour
- The growth kinetic, overproduction of excreted recombinant protein and cell physiology of Pichia pastoris Mut+ strain were studied on mixed substrate containing two carbon sources (glucose and methanol) in chemostat. Although glucose is considered as a repressive substrate for AOX promoter, recombinant trypsinogen was expressed when glucose was used in mixture with methanol in substrate limited culture. The specific methanol uptake rate was rising with increasing dilution rate reaching maximum 0.090.01 g.gCDW-1.h-1 at 0.15 h-1; both co-substrates were utilised simultaneously and fully. At dilution rates, exceeding 0.15 h-1 the specific methanol uptake rate was decreasing and a residual methanol was detected in the culture broth. On the other hand, the specific glucose uptake rate was increasing within the interval of measured dilution rates reaching maximum (0.240.01) g.gCDW-1.h-1 at 0.2 h-1. In case of continuous cultures performed at higher dilution rates the yeasts preferred the higher carbon sour (en)
- Kinetika růstu, nadprodukce rekombinantního proteinu a fyziologie kvasinek Pichia pastoris Mut+ byly sledována na směsném substrátu obsahujícím 2 zdroje uhlíku (glukosu a methanol) rostoucí v kontinuální kultuře. Specificá rychlost spotřeby methanolu vzrůstala se zvyšující se zřeďovací rychlostí, maximum 0.090.01 g.gCDW-1.h-1 bylo dosaženo při D = 0.15 h-1; oba ko-substraty byly utilizovány současně a zcela. Maximální koncentrace trypsinogenu 145 mg.L-1 a maximální specifická productivita (1.17 mg.gCDW-1.h-1) byly dosaženy při D = 0.13 h-1. (cs)
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| Title
| - Production of heterologous protein by Pichia pastoris Mut+ strain grown in continuous culture
- Produkce rekombinantních proteinů buňkami Picha pastoris Mut+ rostoucími v kontinuální kultuře (cs)
- Production of heterologous protein by Pichia pastoris Mut+ strain grown in continuous culture (en)
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| skos:prefLabel
| - Production of heterologous protein by Pichia pastoris Mut+ strain grown in continuous culture
- Produkce rekombinantních proteinů buňkami Picha pastoris Mut+ rostoucími v kontinuální kultuře (cs)
- Production of heterologous protein by Pichia pastoris Mut+ strain grown in continuous culture (en)
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| skos:notation
| - RIV/60461373:22330/08:00021237!RIV09-MSM-22330___
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| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(1P05OE189), Z(MSM6046137305)
|
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/60461373:22330/08:00021237
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - Heterologous protein; Pichia pastoris Continuous culture; Yeast physiology; Flow cytometry (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...ontrolniKodProRIV
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| http://linked.open...v/mistoKonaniAkce
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| http://linked.open...i/riv/mistoVydani
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| http://linked.open...i/riv/nazevZdroje
| - Proceedings of the 13th International Biotechnology Symposium and Exhibition
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...iv/tvurceVysledku
| - Melzoch, Karel
- Paulová, Leona
- Rychtera, Mojmír
- Branská, Barbora
- Vilčková, Stanislava
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| http://linked.open...vavai/riv/typAkce
| |
| http://linked.open.../riv/zahajeniAkce
| |
| http://linked.open...n/vavai/riv/zamer
| |
| issn
| |
| number of pages
| |
| http://purl.org/ne...btex#hasPublisher
| - Dalian Institute of Chemical Physics
|
| http://localhost/t...ganizacniJednotka
| |
| is http://linked.open...avai/riv/vysledek
of | |
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