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  • The aim of this work was to compare batch and continuous hydrolysis of ovine whey proteins in a membrane reactor and to evaluate influence of thermocalcic precipitation before hydrolysis. Reconstituted dried ovine whey fromlump cheese production (a traditional Slovak cheese) was used as a substrate for hydrolysis by Promod 439L protease from Bacillus licheniformis. Initial substrate had 2% proteins andpH8.5. Hydrolysis was performed directly (i) or after a pre-treatment (ii) comprising thermocalcic precipitation at 50 C followed by microfiltration at 40 C, batch-wise or in a membrane ultrafiltration (UF) reactor equipped with 8 kDa ceramic membrane. Mean residence time of 60 min was maintained after batch pre-hydrolysis of the substrate in the first 60 min period, during which the permeate was recirculated. During the subsequent UF, the reaction volumewas kept constant by a continuous feeding of the substrate. After the pre-treatment (ii), crude protein decreased by 25%. Degree of hydrolysis was 9.2%
  • The aim of this work was to compare batch and continuous hydrolysis of ovine whey proteins in a membrane reactor and to evaluate influence of thermocalcic precipitation before hydrolysis. Reconstituted dried ovine whey fromlump cheese production (a traditional Slovak cheese) was used as a substrate for hydrolysis by Promod 439L protease from Bacillus licheniformis. Initial substrate had 2% proteins andpH8.5. Hydrolysis was performed directly (i) or after a pre-treatment (ii) comprising thermocalcic precipitation at 50 C followed by microfiltration at 40 C, batch-wise or in a membrane ultrafiltration (UF) reactor equipped with 8 kDa ceramic membrane. Mean residence time of 60 min was maintained after batch pre-hydrolysis of the substrate in the first 60 min period, during which the permeate was recirculated. During the subsequent UF, the reaction volumewas kept constant by a continuous feeding of the substrate. After the pre-treatment (ii), crude protein decreased by 25%. Degree of hydrolysis was 9.2% (en)
  • Cielom tejto práce bolo porovnanie vsádkovej a kontinuálnej hydrolýzy proteínov ovčej srvátky v membránovom reaktore a ohodnotiť vplyv termokalciovej precipitácie pred hydrolýzou. Obnovená sušená ovčia srvátka z výroby hrudky (tradičný slovenský syr) bola použitá ako substrát pre hydrolýzu s proteázou Promod 439L z Bacillus licheniformis.Východzí substrát obsahoval 2 % proteínov o ph 8.5. Hydrolýza bola robená buď priamo (i) alebo po ošetrení (ii) zahrňajúce termokalciovu precipitáciu pri 50C nasledovanou mikrofiltráciou pri 40C, vsádkovo alebo v membránovom ultrafiltračnom (UF) reaktore vybavenom s 8 kDa keramickou membránou. Doba zdržanie- 60 min bola zachovaná po vsádkovej predhydrolýze substrátu v prvých 60 min, v priebehu ktorých permeát recilkuroval. V priebehu následnej UF, reakčný objem bol držaný konštantne pomocou kontinuálne pridávaneho substrátu. Po ošetrní (ii), sa obsah proteínu znížil o 25%. Stupeň hydrolýzy bol 9.2% (i) a 8.8% (ii) po 60 min pri vsádkovej hydrolýze, a 10.0% (i) a 9.0% (cs)
Title
  • Batch and continuous hydrolysis of ovine whey proteins
  • Vsádková a kontinuální hydrolýza proteinů ovčí syrovátky (cs)
  • Batch and continuous hydrolysis of ovine whey proteins (en)
skos:prefLabel
  • Batch and continuous hydrolysis of ovine whey proteins
  • Vsádková a kontinuální hydrolýza proteinů ovčí syrovátky (cs)
  • Batch and continuous hydrolysis of ovine whey proteins (en)
skos:notation
  • RIV/60461373:22330/08:00021224!RIV09-MSM-22330___
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  • RIV/60461373:22330/08:00021224
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  • ovine whey; protein; hydrolysis; thermocalcic precipitation (en)
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  • BE - Belgické království
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  • Small Ruminant Research
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  • Čurda, Ladislav
  • Jelen, Pavel
  • Mišún, Daniel
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  • 000259884800009
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  • 22330
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