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| Description
| - Equol je fyziologicky významný metabolit vznikající působením dosud neidentifikovaných střevních bakterií na daidzein a formononetin ? isoflavony vyskytující se v soje a dalších bobovitých rostlinách. Vykazuje estrogenní aktivitu, která je dokonce silnější než u jeho prekursorů. Přeměna daidzeinu na equol probíhá pouze u 30-50% lidské populace. V této studii jsme vyvinuli metody nepřímé kompetitivní ELISA pro stanovení equolu v biologickém materiálu (krev, moč). Jako imunogeny byly použity konjugáty equolu s hovězím sérovým albuminem vázané na nosič prostřednictvím karboxymethyletherových můstků v polohách C7 a C4?. Konjugáty stejných haptenů s ovalbuminem byly použity k imobilizaci. Jednotlivé ELISA metody byly optimalizovány testováním různých koncentrací protilátek a potahovacích antigenů, upřesněním reakčního času a využitím blokovacích činidel. Křížové reakce jednotlivých imunoanalytických metod s dalšími isoflavony jsou v přijatelném rozmezí (do 0.6%) a ovlinění molekulami s jinou strukturou j (cs)
- Equol is estrogenic isoflavan, which is formed by the action of intestinal microflora from daidzein (an isoflavone) present in different beverages of leguminous origin. The ability of individual subjects to convert daidzein to equol is highly variable. It has been assumed that equol may have higher biological activity than its precursor. High urinary excretion of equol has been suggested to be associated with a reduction in breast cancer risk and the menopause associated osteoporosis. Here we present the development of a competitive enzyme-linked immunosorbent assay (ELISA) method for the determination of equol in human body fluids. Isoflavone conjugates coupled to bovine serum albumin at positions 7-O- and 4´-O- via a carboxymethyl spacer were used as immunogens. Conjugates of the same haptens with ovalbumin have been synthesized as coating antigens. Assay?s conditions, including concentrations of antisera and coating antigens, were optimized and the effect of reaction time was determined, various
- Equol is estrogenic isoflavan, which is formed by the action of intestinal microflora from daidzein (an isoflavone) present in different beverages of leguminous origin. The ability of individual subjects to convert daidzein to equol is highly variable. It has been assumed that equol may have higher biological activity than its precursor. High urinary excretion of equol has been suggested to be associated with a reduction in breast cancer risk and the menopause associated osteoporosis. Here we present the development of a competitive enzyme-linked immunosorbent assay (ELISA) method for the determination of equol in human body fluids. Isoflavone conjugates coupled to bovine serum albumin at positions 7-O- and 4´-O- via a carboxymethyl spacer were used as immunogens. Conjugates of the same haptens with ovalbumin have been synthesized as coating antigens. Assay?s conditions, including concentrations of antisera and coating antigens, were optimized and the effect of reaction time was determined, various (en)
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| Title
| - ELISA method for estimation of equol
- ELISA method for estimation of equol (en)
- ELISA pro stanovení equolu (cs)
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| skos:prefLabel
| - ELISA method for estimation of equol
- ELISA method for estimation of equol (en)
- ELISA pro stanovení equolu (cs)
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| skos:notation
| - RIV/60461373:22330/08:00019961!RIV09-MSM-22330___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GA303/08/0958), Z(MSM6046137305)
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/60461373:22330/08:00019961
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - equol; phytoestrogen; ELISA (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...v/mistoKonaniAkce
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| http://linked.open...i/riv/mistoVydani
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| http://linked.open...i/riv/nazevZdroje
| - Polyphenols Communications
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...iv/tvurceVysledku
| - Lapčík, Oldřich
- Al-Maharik, Nawaf
- Lanková, Petra
- Bukáčková, Lucie
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| http://linked.open...vavai/riv/typAkce
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| http://linked.open.../riv/zahajeniAkce
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| http://linked.open...n/vavai/riv/zamer
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| number of pages
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| http://purl.org/ne...btex#hasPublisher
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| https://schema.org/isbn
| |
| http://localhost/t...ganizacniJednotka
| |
| is http://linked.open...avai/riv/vysledek
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