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| Description
| - Corynebacterium glutamicum R adhA gene encodes a homodimeric, NAD-dependent, 345-amino acid resudue alcohol dehydrogenase with two zinc ions per subunit. Chromosomal inactivation of the adhA gene rendered the strain incapable of growth on either ethanol or n-propanol as the sole carbon source. RNA hybridization analysis revealed that adhA transcription was not only induced by these two substrates, but it was also subject to glucose catabolite repression. Accordingly, both induction of AdhA activity and ethanol utilization were detected only after depletion of glucose. Deletion of either or both of potential cAMP receptor binding site (CBS) and an inverted repeat (IR) of sequence 5-GCAATTGATG-N8-CACAATTGC-3 in the promoter region of adhA strongly suggested that IR, which does not share significant similarity with other regulatory DNA elements of C. glutamicum, represents a transcriptional repressor binding site. Purified recombinant AdhA displayed the highest substrate specificities towa
- Corynebacterium glutamicum R adhA gene encodes a homodimeric, NAD-dependent, 345-amino acid resudue alcohol dehydrogenase with two zinc ions per subunit. Chromosomal inactivation of the adhA gene rendered the strain incapable of growth on either ethanol or n-propanol as the sole carbon source. RNA hybridization analysis revealed that adhA transcription was not only induced by these two substrates, but it was also subject to glucose catabolite repression. Accordingly, both induction of AdhA activity and ethanol utilization were detected only after depletion of glucose. Deletion of either or both of potential cAMP receptor binding site (CBS) and an inverted repeat (IR) of sequence 5-GCAATTGATG-N8-CACAATTGC-3 in the promoter region of adhA strongly suggested that IR, which does not share significant similarity with other regulatory DNA elements of C. glutamicum, represents a transcriptional repressor binding site. Purified recombinant AdhA displayed the highest substrate specificities towa (en)
- Gen adhA z Corynebacterium glutamicum R kóduje homodimerní, NAD-dependentní, alkoholdehydrogenasu o 345 aminokyselinových zbytcích, jejíž podjednotka obsahuje 2 zinečnaté ionty. Chromosomální inaktivace adhA vedla ke kmeni, který ztratil schopnost růstu na ethanolu a n-propanolu jako zdrojích uhlíku. Hybridizační studie ukázaly, že transkripce adhA je indukována jen těmito alkoholy a podléhá katabolické represi glukosou. Jak indukce aktivity AdhA, tak utilizace ethanolu byly detekovány pouze poté, co byla veškerá glukosa z media spotřebována. Delece v promotorové oblasti adhA eliminující potencionální vazebné místo pro cAMP Receptor Protein (CBS), inverzně repeptitivní (IR) sekvenci 5'-GCAATTGATG-N8-CACAATTGC-3', nebo oba elementy současně ukázaly, že IR představuje vazebné místo pro transkripční repressor. Purifikovaná AdhA vykazovala nejvyšší substrátové specifity k ethanolu, n-propanolu a k odpovídajícím aldehydům (cs)
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| Title
| - Transcriptionally regulated adhA gene encodes alcohol dehydrogenase required for ethanol and n-propanol utilization in Corynebacterium glutamicum R
- Transkripčně regulovaný gen adhA kóduje alkoholdehydrogenasu nezbytnou pro utilizaci ethanolu a n-propanolu v Corynebacterium glutamicum R (cs)
- Transcriptionally regulated adhA gene encodes alcohol dehydrogenase required for ethanol and n-propanol utilization in Corynebacterium glutamicum R (en)
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| skos:prefLabel
| - Transcriptionally regulated adhA gene encodes alcohol dehydrogenase required for ethanol and n-propanol utilization in Corynebacterium glutamicum R
- Transkripčně regulovaný gen adhA kóduje alkoholdehydrogenasu nezbytnou pro utilizaci ethanolu a n-propanolu v Corynebacterium glutamicum R (cs)
- Transcriptionally regulated adhA gene encodes alcohol dehydrogenase required for ethanol and n-propanol utilization in Corynebacterium glutamicum R (en)
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| skos:notation
| - RIV/60461373:22330/07:00019140!RIV08-MSM-22330___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/60461373:22330/07:00019140
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Alkoholdehydrogenasa; ethanol; Corynebacterium glutamicum; katabolická represe (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Applied Microbiology and Biotechnology
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Kotrba, Pavel
- Sajdok, Jiří
- Kotrbová-Kozak, Anna Katarzyna
- Hideaki, Yukawa
- Masayuki, Inui
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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| is http://linked.open...avai/riv/vysledek
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