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Description
  • Byla studována struktura caleosinu (cs)
  • We have investigated the covalent and secondary solution structure of caleosin, a 27kDa protein also called ATS1 or AtClo1 (At4g26740) found within Arabidopsis thaliana seed lipid bodies. The native protein was partly phosphorylated at S225. Purified bacterially expressed caleosin (recClo) was not phosphorylated; cysteine residues C221 and C230 were connected by a disulfide bridge. In solution it exists as a mixture of predominant monomers and covalent dimers. We have used recClo as a model for the study of AtClo1 secondary structure. recClo is folded in aqueous solution (16% alpha helix, 29% beta sheet), its secondary structure being dramatically influenced by the polarity of media, as deduced from CD spectra measured in the presence of increasing concentrations of various aliphatic alcohols.
  • We have investigated the covalent and secondary solution structure of caleosin, a 27kDa protein also called ATS1 or AtClo1 (At4g26740) found within Arabidopsis thaliana seed lipid bodies. The native protein was partly phosphorylated at S225. Purified bacterially expressed caleosin (recClo) was not phosphorylated; cysteine residues C221 and C230 were connected by a disulfide bridge. In solution it exists as a mixture of predominant monomers and covalent dimers. We have used recClo as a model for the study of AtClo1 secondary structure. recClo is folded in aqueous solution (16% alpha helix, 29% beta sheet), its secondary structure being dramatically influenced by the polarity of media, as deduced from CD spectra measured in the presence of increasing concentrations of various aliphatic alcohols. (en)
Title
  • Structural properties of caleosin: A MS and CD study
  • Structurní vlastnosti caleosinu: MS a CD studie (cs)
  • Structural properties of caleosin: A MS and CD study (en)
skos:prefLabel
  • Structural properties of caleosin: A MS and CD study
  • Structurní vlastnosti caleosinu: MS a CD studie (cs)
  • Structural properties of caleosin: A MS and CD study (en)
skos:notation
  • RIV/60461373:22330/07:00019129!RIV08-MSM-22330___
http://linked.open.../vavai/riv/strany
  • 335-343
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM6046137305)
http://linked.open...iv/cisloPeriodika
  • 2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 452873
http://linked.open...ai/riv/idVysledku
  • RIV/60461373:22330/07:00019129
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Claeosin; lipid bodies; phosphorylation; circular dichroism (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [519139507CA6]
http://linked.open...i/riv/nazevZdroje
  • Archives of Biochemistry and Biophysics
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 464
http://linked.open...iv/tvurceVysledku
  • Lipovová, Petra
  • Králová, Blanka
  • Purkrtová, Zita
  • Chardot, Thiery
  • D'andrea, Sabine
  • Jolivet, Pascal
http://linked.open...n/vavai/riv/zamer
issn
  • 0003-9861
number of pages
http://localhost/t...ganizacniJednotka
  • 22330
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