About: New Method of Age Estimation based on the Changes in Human Non-collagenous Proteins from Dentin     Goto   Sponge   NotDistinct   Permalink

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Description
  • The new method exploits the characteristic of staphylococcal protease V8, which specifically splits peptide bonds where l-glutamic or l-aspartic acid participate. Those peptide bonds where d-aspartic acid is present remain unsplit because of the stereospecifity of enzymes. In accordance with expectations, fewer peptide bonds are split by this protease at more advanced ages and larger peptide fragments are thus formed due to the higher content of d-amino acid residues in the proteins of older people. The samples of acid-extracted non-collagenous proteins from dentin were separated using high performance liquid chromatography after enzymatic hydrolysis. A peak with a retention time of 45.3 min was chosen and his enlarging area showed a linear correlation with increasing age. Although the linear correlation with age was proved, the scattering of values decreases the usefulness of the proposed method for age estimation.
  • The new method exploits the characteristic of staphylococcal protease V8, which specifically splits peptide bonds where l-glutamic or l-aspartic acid participate. Those peptide bonds where d-aspartic acid is present remain unsplit because of the stereospecifity of enzymes. In accordance with expectations, fewer peptide bonds are split by this protease at more advanced ages and larger peptide fragments are thus formed due to the higher content of d-amino acid residues in the proteins of older people. The samples of acid-extracted non-collagenous proteins from dentin were separated using high performance liquid chromatography after enzymatic hydrolysis. A peak with a retention time of 45.3 min was chosen and his enlarging area showed a linear correlation with increasing age. Although the linear correlation with age was proved, the scattering of values decreases the usefulness of the proposed method for age estimation. (en)
  • The new method exploits the characteristic of staphylococcal protease V8, which specifically splits peptide bonds where l-glutamic or l-aspartic acid participate. Those peptide bonds where d-aspartic acid is present remain unsplit because of the stereospecifity of enzymes. In accordance with expectations, fewer peptide bonds are split by this protease at more advanced ages and larger peptide fragments are thus formed due to the higher content of d-amino acid residues in the proteins of older people. The samples of acid-extracted non-collagenous proteins from dentin were separated using high performance liquid chromatography after enzymatic hydrolysis. A peak with a retention time of 45.3 min was chosen and his enlarging area showed a linear correlation with increasing age. Although the linear correlation with age was proved, the scattering of values decreases the usefulness of the proposed method for age estimation. (cs)
Title
  • New Method of Age Estimation based on the Changes in Human Non-collagenous Proteins from Dentin
  • New Method of Age Estimation based on the Changes in Human Non-collagenous Proteins from Dentin (en)
  • New Method of Age Estimation based on the Changes in Human Non-collagenous Proteins from Dentin (cs)
skos:prefLabel
  • New Method of Age Estimation based on the Changes in Human Non-collagenous Proteins from Dentin
  • New Method of Age Estimation based on the Changes in Human Non-collagenous Proteins from Dentin (en)
  • New Method of Age Estimation based on the Changes in Human Non-collagenous Proteins from Dentin (cs)
skos:notation
  • RIV/60461373:22330/06:00017745!RIV07-MSM-22330___
http://linked.open.../vavai/riv/strany
  • 245-249
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM6046137305)
http://linked.open...iv/cisloPeriodika
  • 156
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 488539
http://linked.open...ai/riv/idVysledku
  • RIV/60461373:22330/06:00017745
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Non-collagenous Proteins (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [FB23DDE9A5EF]
http://linked.open...i/riv/nazevZdroje
  • Forensic Science International
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Káš, Jan
  • Zídková, Jarmila
  • Pudil, František
  • Sajdok, Jiří
  • Pilin, Alexandr
http://linked.open...n/vavai/riv/zamer
issn
  • 0379-0738
number of pages
http://localhost/t...ganizacniJednotka
  • 22330
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