About: Development of a rapid, simple paddle-style dipstick dye immunoassay specific for Listeria monocytogenes

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Byly vyvinuty dva typy dipstikových imunoanalýz. Jedna je specifická pro Listérie a druhá pro Listeria monocytogenes. (cs) We have developed two types of new paddlestyle dipstick dye immunoassays. The first is genus Listeria specific and the second is specific to Listeria monocytogenes. They are based respectively, on antisera raised against heat-killed L. monocytogenes cells and against internalin B crude extract, a virulence protein found only in the pathogenic L. monocytogenes. The minimum detectable level for L. monocytogenes is 2x107 CFU ml−1 for strain number 88/049 in pure culture. Detection is unaffected by the presence of high numbers (approximately log 8.0 CFU/ml) of the other microorganisms tested. When the dipsticks were applied to milk samples inoculated with L. monocytogenes reference material (ALM92), there was a strong response to the enrichment cultures. The new assay may prove useful in detection of L. monocytogenes in enrichment cultures of milk and ice cream food samples. We have developed two types of new paddlestyle dipstick dye immunoassays. The first is genus Listeria specific and the second is specific to Listeria monocytogenes. They are based respectively, on antisera raised against heat-killed L. monocytogenes cells and against internalin B crude extract, a virulence protein found only in the pathogenic L. monocytogenes. The minimum detectable level for L. monocytogenes is 2x107 CFU ml−1 for strain number 88/049 in pure culture. Detection is unaffected by the presence of high numbers (approximately log 8.0 CFU/ml) of the other microorganisms tested. When the dipsticks were applied to milk samples inoculated with L. monocytogenes reference material (ALM92), there was a strong response to the enrichment cultures. The new assay may prove useful in detection of L. monocytogenes in enrichment cultures of milk and ice cream food samples. (en)
Title	Development of a rapid, simple paddle-style dipstick dye immunoassay specific for Listeria monocytogenes Vývoj rychlé a jednoduché imunoanalýzy specifické pro Listeria monocytogenes s vyuřitím dipstiků (cs) Development of a rapid, simple paddle-style dipstick dye immunoassay specific for Listeria monocytogenes (en)
skos:prefLabel	Development of a rapid, simple paddle-style dipstick dye immunoassay specific for Listeria monocytogenes Vývoj rychlé a jednoduché imunoanalýzy specifické pro Listeria monocytogenes s vyuřitím dipstiků (cs) Development of a rapid, simple paddle-style dipstick dye immunoassay specific for Listeria monocytogenes (en)
skos:notation	RIV/60461373:22330/06:00017221!RIV07-MSM-22330___
http://linked.open.../vavai/riv/strany	821-827
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA525/03/0350), Z(MSM6046137305)
http://linked.open...iv/cisloPeriodika	223
http://linked.open...vai/riv/dodaniDat	2007
http://linked.open...aciTvurceVysledku	Karasová, Ludmila Fukal, Ladislav Blažková, Martina Rauch, Pavel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta potravinářské a biochemické technologie
http://linked.open...dnocenehoVysledku	471272
http://linked.open...ai/riv/idVysledku	RIV/60461373:22330/06:00017221
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	dipstick immunoassay; Listeia monocytogenes; milk; ice cream (en)
http://linked.open.../riv/klicoveSlovo	milk ice cream Listeia monocytogenes dipstick immunoassay
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[B1A2F81EE94A]
http://linked.open...i/riv/nazevZdroje	European Food Research and Technology
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Development of screening immunochemical method for specific detection of Listeria monocytogenes in foods
http://linked.open...UplatneniVysledku	2006
http://linked.open...iv/tvurceVysledku	Blažková, Martina Fukal, Ladislav Hoza, Ignác Rauch, Pavel Karamonová, Ludmila Wyatt, Gary Greifová, Magda
http://linked.open...n/vavai/riv/zamer	Teoretické základy potravinářských a biochemických technologií
issn	1438-2377
number of pages	7 (xsd:int)
http://localhost/t...ganizacniJednotka	22330
is http://linked.open...avai/riv/vysledek of	Development of a rapid, simple paddle-style dipstick dye immunoassay specific for Listeria monocytogenes Development of a rapid, simple paddle-style dipstick dye immunoassay specific for Listeria monocytogenes

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