About: Analytical performances of validated chemiluminescent enzyme immunoassays to detect N-methylcarbamate pesticides.

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In the present work, the comparison study of enzyme-linked immunosorbent assays (ELISAs) with colorimetric detection and ELISAs with chemiluminescent detection for the determination of carbofuran, carbaryl and methiocarb is described. Both conjugate-coated formats are based on identical monoclonal antibodies and homologous protein conjugates. The sensitivity of immunochemical method was expressed as a detection limit, a linear working range and an I50 value. In comparison with colorimetric ELISA, the ability of the chemiluminescent technique to detect lower concentration of HRP allowed to decreased the optimal antibody and conjugate concentrations and thus to reach lower analytical parameters. The experimental comparison of the analytical performance of ELISAs was carried out by simultaneous analysis of apple-strawberry baby food extracts spiked with mentioned pesticides at three different concentration levels. The extracts were prepared in two duplicate sets - crude extracts and extracts purified by In the present work, the comparison study of enzyme-linked immunosorbent assays (ELISAs) with colorimetric detection and ELISAs with chemiluminescent detection for the determination of carbofuran, carbaryl and methiocarb is described. Both conjugate-coated formats are based on identical monoclonal antibodies and homologous protein conjugates. The sensitivity of immunochemical method was expressed as a detection limit, a linear working range and an I50 value. In comparison with colorimetric ELISA, the ability of the chemiluminescent technique to detect lower concentration of HRP allowed to decreased the optimal antibody and conjugate concentrations and thus to reach lower analytical parameters. The experimental comparison of the analytical performance of ELISAs was carried out by simultaneous analysis of apple-strawberry baby food extracts spiked with mentioned pesticides at three different concentration levels. The extracts were prepared in two duplicate sets - crude extracts and extracts purified by (en)
Title	Analytical performances of validated chemiluminescent enzyme immunoassays to detect N-methylcarbamate pesticides. Analytical performances of validated chemiluminescent enzyme immunoassays to detect N-methylcarbamate pesticides. (en)
skos:prefLabel	Analytical performances of validated chemiluminescent enzyme immunoassays to detect N-methylcarbamate pesticides. Analytical performances of validated chemiluminescent enzyme immunoassays to detect N-methylcarbamate pesticides. (en)
skos:notation	RIV/60461373:22330/05:00022532!RIV10-MSM-22330___
http://linked.open...avai/riv/aktivita	S
http://linked.open...avai/riv/aktivity	S
http://linked.open...iv/cisloPeriodika	528
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Holubová, Barbora Rauch, Pavel Kovalczuk, Tomáš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta potravinářské a biochemické technologie
http://linked.open...dnocenehoVysledku	512175
http://linked.open...ai/riv/idVysledku	RIV/60461373:22330/05:00022532
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	N-methylcarbamate insecticides; baby food; ELISA; HPLC/MS/MS; colorimetric detection; chemiluminescent detection (en)
http://linked.open.../riv/klicoveSlovo	colorimetric detection baby food N-methylcarbamate insecticides ELISA chemiluminescent detection HPLC/MS/MS
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[2633597FA011]
http://linked.open...i/riv/nazevZdroje	Anal.Chim.Acta
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...UplatneniVysledku	2005
http://linked.open...v/svazekPeriodika	neuveden
http://linked.open...iv/tvurceVysledku	Rauch, Pavel Kovalczuk, Tomáš Holubová, Roz.mičková, Barbora Fini, Fabiana Abad, Antonio Montoya, Angel Moreno, Maria-Jose Girotti, Stephano Ferri, Elida
http://linked.open...ain/vavai/riv/wos	000226455800013
issn	0003-2670
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	22330
is http://linked.open...avai/riv/vysledek of	Analytical performances of validated chemiluminescent enzyme immunoassays to detect N-methylcarbamate pesticides.

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