About: Cage-like Hexamers of Cold-active beta-galactosidase from Arthrobacter sp. C2-2.     Goto   Sponge   NotDistinct   Permalink

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Description
  • #Ŕ-galactosidase catalyzes hydrolysis of galactosyl moiety fromnon-reducing termini of oligosaccharides or from glycosides. Crystalsof #Ŕ-galactosidase from psychrotrophic bacterium Arthrobacter sp.C2-2 were grown by vapor diffusion technique and X-ray diffractiondata were collected up to 1.9 A [1]. Molecular replacement solution inP21 space group revealed six molecules arranged in cage-likehexameric structure with cca 60,000 non-hydrogen atoms perasymmetric unit. The compact hexamers are characterized by threetypes of channels, six active sites open towards the central cavity, highnumber of buried water molecules within the protein and Na+ andMg2+ ions bound in vicinity of the active site. Comparison to E.coli #Ŕ-galactosidase shows both similarities and significant differencesregarding the active site and oligomerization mechanism.Acknowledgement: This work was supported by GACR (project204/02/0843/A) and by the Grant Agency of the Academy of Sciencesof the Czech Republic (project KJB500500512).
  • #Ŕ-galactosidase catalyzes hydrolysis of galactosyl moiety fromnon-reducing termini of oligosaccharides or from glycosides. Crystalsof #Ŕ-galactosidase from psychrotrophic bacterium Arthrobacter sp.C2-2 were grown by vapor diffusion technique and X-ray diffractiondata were collected up to 1.9 A [1]. Molecular replacement solution inP21 space group revealed six molecules arranged in cage-likehexameric structure with cca 60,000 non-hydrogen atoms perasymmetric unit. The compact hexamers are characterized by threetypes of channels, six active sites open towards the central cavity, highnumber of buried water molecules within the protein and Na+ andMg2+ ions bound in vicinity of the active site. Comparison to E.coli #Ŕ-galactosidase shows both similarities and significant differencesregarding the active site and oligomerization mechanism.Acknowledgement: This work was supported by GACR (project204/02/0843/A) and by the Grant Agency of the Academy of Sciencesof the Czech Republic (project KJB500500512). (en)
  • Klecovité hexamery chladově-aktivní beta-galaktosidasy z Arthrobacter sp. C2-2, (cs)
Title
  • Cage-like Hexamers of Cold-active beta-galactosidase from Arthrobacter sp. C2-2.
  • Cage-like Hexamers of Cold-active beta-galactosidase from Arthrobacter sp. C2-2. (en)
  • Klecovité hexamery chladově-aktivní beta-galaktosidasy z Arthrobacter sp. C2-2 (cs)
skos:prefLabel
  • Cage-like Hexamers of Cold-active beta-galactosidase from Arthrobacter sp. C2-2.
  • Cage-like Hexamers of Cold-active beta-galactosidase from Arthrobacter sp. C2-2. (en)
  • Klecovité hexamery chladově-aktivní beta-galaktosidasy z Arthrobacter sp. C2-2 (cs)
skos:notation
  • RIV/60461373:22330/05:00015988!RIV06-GA0-22330___
http://linked.open.../vavai/riv/strany
  • 237-237
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA204/02/0843), Z(MSM 223300006)
http://linked.open...iv/cisloPeriodika
  • A61
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 514351
http://linked.open...ai/riv/idVysledku
  • RIV/60461373:22330/05:00015988
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • cold-active; beta-galactosidase; crystal structure (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [1915C0AAC633]
http://linked.open...i/riv/nazevZdroje
  • Acta Crystallogr., Sect.A: Found.Crystallogr.
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • A61
http://linked.open...iv/tvurceVysledku
  • Dohnálek, Jan
  • Dušková, Jarmila
  • Hašek, Jindřich
  • Lipovová, Petra
  • Skálová, Tereza
  • Spiwok, Vojtěch
  • Králová, Blanka
  • Petroková, Hana
  • Buchtelová, Eva
http://linked.open...n/vavai/riv/zamer
issn
  • 0108-7676
number of pages
http://localhost/t...ganizacniJednotka
  • 22330
is http://linked.open...avai/riv/vysledek of
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