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rdf:type
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Description
| - #Ŕ-galactosidase catalyzes hydrolysis of galactosyl moiety fromnon-reducing termini of oligosaccharides or from glycosides. Crystalsof #Ŕ-galactosidase from psychrotrophic bacterium Arthrobacter sp.C2-2 were grown by vapor diffusion technique and X-ray diffractiondata were collected up to 1.9 A [1]. Molecular replacement solution inP21 space group revealed six molecules arranged in cage-likehexameric structure with cca 60,000 non-hydrogen atoms perasymmetric unit. The compact hexamers are characterized by threetypes of channels, six active sites open towards the central cavity, highnumber of buried water molecules within the protein and Na+ andMg2+ ions bound in vicinity of the active site. Comparison to E.coli #Ŕ-galactosidase shows both similarities and significant differencesregarding the active site and oligomerization mechanism.Acknowledgement: This work was supported by GACR (project204/02/0843/A) and by the Grant Agency of the Academy of Sciencesof the Czech Republic (project KJB500500512).
- #Ŕ-galactosidase catalyzes hydrolysis of galactosyl moiety fromnon-reducing termini of oligosaccharides or from glycosides. Crystalsof #Ŕ-galactosidase from psychrotrophic bacterium Arthrobacter sp.C2-2 were grown by vapor diffusion technique and X-ray diffractiondata were collected up to 1.9 A [1]. Molecular replacement solution inP21 space group revealed six molecules arranged in cage-likehexameric structure with cca 60,000 non-hydrogen atoms perasymmetric unit. The compact hexamers are characterized by threetypes of channels, six active sites open towards the central cavity, highnumber of buried water molecules within the protein and Na+ andMg2+ ions bound in vicinity of the active site. Comparison to E.coli #Ŕ-galactosidase shows both similarities and significant differencesregarding the active site and oligomerization mechanism.Acknowledgement: This work was supported by GACR (project204/02/0843/A) and by the Grant Agency of the Academy of Sciencesof the Czech Republic (project KJB500500512). (en)
- Klecovité hexamery chladově-aktivní beta-galaktosidasy z Arthrobacter sp. C2-2, (cs)
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Title
| - Cage-like Hexamers of Cold-active beta-galactosidase from Arthrobacter sp. C2-2.
- Cage-like Hexamers of Cold-active beta-galactosidase from Arthrobacter sp. C2-2. (en)
- Klecovité hexamery chladově-aktivní beta-galaktosidasy z Arthrobacter sp. C2-2 (cs)
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skos:prefLabel
| - Cage-like Hexamers of Cold-active beta-galactosidase from Arthrobacter sp. C2-2.
- Cage-like Hexamers of Cold-active beta-galactosidase from Arthrobacter sp. C2-2. (en)
- Klecovité hexamery chladově-aktivní beta-galaktosidasy z Arthrobacter sp. C2-2 (cs)
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skos:notation
| - RIV/60461373:22330/05:00015988!RIV06-GA0-22330___
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(GA204/02/0843), Z(MSM 223300006)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/60461373:22330/05:00015988
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - cold-active; beta-galactosidase; crystal structure (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Acta Crystallogr., Sect.A: Found.Crystallogr.
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Dohnálek, Jan
- Dušková, Jarmila
- Hašek, Jindřich
- Lipovová, Petra
- Skálová, Tereza
- Spiwok, Vojtěch
- Králová, Blanka
- Petroková, Hana
- Buchtelová, Eva
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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http://localhost/t...ganizacniJednotka
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is http://linked.open...avai/riv/vysledek
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