About: Cloning of bacterial PCB degradative genes to plants

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Vložení bakteriálního genu degraduící PCB do rostlin (cs) ##The target of this work is to increase biodegradation of polychlorinated biphenyls (PCBs) by the plants transformed with the gene from bacterial PCB degradation pathway. Bacterial aerobic PCB degradation pathway contains 4 steps (bph operon) in which the third one (bphC) catalyzes the conversion of 2,3-dihydroxybiphenyl into 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid and thus destroyes the biphenyl ring. For this purpose the bphC gene encoding the enzyme 2,3-dihydroxybiphenyl-1,2-dioxygenase was chosen from bacteria Pseudomonas testosteroni B-356 for cloning into plant Nicotiana tabacum. Different constructs in cassette with bphC containing genes for GFP (green fluorescent protein), GUS (glucuronidase) and LUC (luciferase) were prepared to allow sensitive detection of bphC expression in plants. Also construct in which bphC was joined to His tail was designed. The constructs with bphC/GFP was already transformed into the Nicotiana tabacum. The presence of bphC/GFP DNA and RNA was proved by PCR an ##The target of this work is to increase biodegradation of polychlorinated biphenyls (PCBs) by the plants transformed with the gene from bacterial PCB degradation pathway. Bacterial aerobic PCB degradation pathway contains 4 steps (bph operon) in which the third one (bphC) catalyzes the conversion of 2,3-dihydroxybiphenyl into 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid and thus destroyes the biphenyl ring. For this purpose the bphC gene encoding the enzyme 2,3-dihydroxybiphenyl-1,2-dioxygenase was chosen from bacteria Pseudomonas testosteroni B-356 for cloning into plant Nicotiana tabacum. Different constructs in cassette with bphC containing genes for GFP (green fluorescent protein), GUS (glucuronidase) and LUC (luciferase) were prepared to allow sensitive detection of bphC expression in plants. Also construct in which bphC was joined to His tail was designed. The constructs with bphC/GFP was already transformed into the Nicotiana tabacum. The presence of bphC/GFP DNA and RNA was proved by PCR an (en)
Title	Cloning of bacterial PCB degradative genes to plants Cloning of bacterial PCB degradative genes to plants (en) Klonování PCB degradujícího bakteriálního genu do rostlin (cs)
skos:prefLabel	Cloning of bacterial PCB degradative genes to plants Cloning of bacterial PCB degradative genes to plants (en) Klonování PCB degradujícího bakteriálního genu do rostlin (cs)
skos:notation	RIV/60461373:22330/04:00017137!RIV07-MSM-22330___
http://linked.open.../vavai/riv/strany	247-248
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(LN00B030), Z(MSM 223300006)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2007
http://linked.open...aciTvurceVysledku	Nováková, Martina Borovka, Radek Frančová, Kateřina Macková, Martina Macek, Tomáš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta potravinářské a biochemické technologie
http://linked.open...dnocenehoVysledku	557894
http://linked.open...ai/riv/idVysledku	RIV/60461373:22330/04:00017137
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cloning; PCB (en)
http://linked.open.../riv/klicoveSlovo	PCB cloning
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[00822A98A782]
http://linked.open...i/riv/nazevZdroje	Int. Biodeterioration and Biodegradation
http://linked.open...in/vavai/riv/obor	EI
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Center for molecular and gene biotechnologies
http://linked.open...UplatneniVysledku	2004
http://linked.open...v/svazekPeriodika	53
http://linked.open...iv/tvurceVysledku	Macek, Tomáš Macková, Martina Frančová, Kateřina Surá, Martina Szekeres, M. Borovka, Radek Sylvestre, Michel Bankoc, Simona
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20223300006
issn	0964-8305
number of pages	1 (xsd:int)
http://localhost/t...ganizacniJednotka	22330

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