About: The Determination of N-methylcarbamate Pesticides Using Enzyme Immunoassays with Chemiluminescent Detection

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In the present work, enzyme-linked immunosorbent assays (ELISAs) with chemiluminescent detection for the determination of carbofuran, carbaryl and methiocarb were developed and the analytical parameters of these assays were compared with those of ELISAs with colorimetric detection. The sensitivity of immunochemical methods was expressed as detection limit, linear working range, and I50 value. In comparison with colorimetric ELISA, the ability of the chemiluminescent reagents to detect lower concentrations of HRP allowed to decrease the optimal antibody and conjugate concentrations and to reach better analytical parameters. The experimental comparison of the analytical performance of the ELISAs was carried out by analysing simply diluted fruit juices, spiked at different concentration levels with the above mentioned pesticides. Recovery values for both ELISAs were around 100 % and no matrix effects were observed when fruit juices were diluted 1:20 or more. In the present work, enzyme-linked immunosorbent assays (ELISAs) with chemiluminescent detection for the determination of carbofuran, carbaryl and methiocarb were developed and the analytical parameters of these assays were compared with those of ELISAs with colorimetric detection. The sensitivity of immunochemical methods was expressed as detection limit, linear working range, and I50 value. In comparison with colorimetric ELISA, the ability of the chemiluminescent reagents to detect lower concentrations of HRP allowed to decrease the optimal antibody and conjugate concentrations and to reach better analytical parameters. The experimental comparison of the analytical performance of the ELISAs was carried out by analysing simply diluted fruit juices, spiked at different concentration levels with the above mentioned pesticides. Recovery values for both ELISAs were around 100 % and no matrix effects were observed when fruit juices were diluted 1:20 or more. (en) Stanovení N-methylkarbamátových pesticidů pomocí enzymové imunoanalýzy s chemiluminescenční detekcí (cs)
Title	The Determination of N-methylcarbamate Pesticides Using Enzyme Immunoassays with Chemiluminescent Detection The Determination of N-methylcarbamate Pesticides Using Enzyme Immunoassays with Chemiluminescent Detection (en) Stanovení N-methylkarbamátových pesticidů pomocí enzymové imunoanalýzy s chemiluminescenční detekcí (cs)
skos:prefLabel	The Determination of N-methylcarbamate Pesticides Using Enzyme Immunoassays with Chemiluminescent Detection The Determination of N-methylcarbamate Pesticides Using Enzyme Immunoassays with Chemiluminescent Detection (en) Stanovení N-methylkarbamátových pesticidů pomocí enzymové imunoanalýzy s chemiluminescenční detekcí (cs)
skos:notation	RIV/60461373:22330/04:00012904!RIV/2005/MSM/223305/N
http://linked.open.../vavai/riv/strany	280-282
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM 223300006)
http://linked.open...vai/riv/dodaniDat	2005
http://linked.open...aciTvurceVysledku	Holubová, Barbora Rauch, Pavel
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta potravinářské a biochemické technologie
http://linked.open...dnocenehoVysledku	560095
http://linked.open...ai/riv/idVysledku	RIV/60461373:22330/04:00012904
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	ELISA;N-methylcarbamate pesticide;chemiluminescent detection (en)
http://linked.open.../riv/klicoveSlovo	N-methylcarbamate pesticide ELISA chemiluminescent detection
http://linked.open...ontrolniKodProRIV	[D0D36CF7B26A]
http://linked.open...v/mistoKonaniAkce	Praha
http://linked.open...i/riv/mistoVydani	Praha
http://linked.open...i/riv/nazevZdroje	Czech Journal of Food Science
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...UplatneniVysledku	2004
http://linked.open...iv/tvurceVysledku	Rauch, Pavel Fini, Fabiana Mičková, Barbora Montoya, Angel Girotti, Stephano Ferri, Elida
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2004-09-29 (xsd:date)
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20223300006
issn	1212-1800
number of pages	3 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Česká akademie zemědělských věd
http://localhost/t...ganizacniJednotka	22330

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