About: Development of monoclonal immunoassay selective for chlorinated cyclodiene insecticides

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Development of monoclonal immunoassay selective for chlorinated cyclodiene insecticides Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Organochlorine pesticides still generate public health concerns because of their unresolved health impact and their persistence in living beings, which is demanding appropriate analytical techniques for their monitoring. In this study, an enzyme-linked immunosorbent assay based on monoclonal antibodies (MAbs) for the detection of an important group of organochlorine pesticides, the cyclodiene group, has been developed. With this aim, several hapten-protein conjugates, characterized by exposure of the common hexachlorinated bicyclic (norbornene) moiety and differing in the linking structure to the carrier protein, were prepared. From mice immunized with these conjugates, several MAbs with the ability to sensitively bind the majority of cyclodienes were obtained. Among them CCD2.2 MAb displaying the broadest recognition to cyclodiene compounds (endosulfan, dieldrin, endrin, chlordane, heptachlor, aldrin, toxaphene: I50 values in the 6-25 nM range) was selected for the assay. Interestingly, this MAb show Organochlorine pesticides still generate public health concerns because of their unresolved health impact and their persistence in living beings, which is demanding appropriate analytical techniques for their monitoring. In this study, an enzyme-linked immunosorbent assay based on monoclonal antibodies (MAbs) for the detection of an important group of organochlorine pesticides, the cyclodiene group, has been developed. With this aim, several hapten-protein conjugates, characterized by exposure of the common hexachlorinated bicyclic (norbornene) moiety and differing in the linking structure to the carrier protein, were prepared. From mice immunized with these conjugates, several MAbs with the ability to sensitively bind the majority of cyclodienes were obtained. Among them CCD2.2 MAb displaying the broadest recognition to cyclodiene compounds (endosulfan, dieldrin, endrin, chlordane, heptachlor, aldrin, toxaphene: I50 values in the 6-25 nM range) was selected for the assay. Interestingly, this MAb show (en) Vývoj monoklonální imunonometody selektivní pro chlorované cyklodienové insekticidy (cs)
Title	Vývoj monoklonální imunonometody selektivní pro chlorované cyklodienové insekticidy (cs) Development of monoclonal immunoassay selective for chlorinated cyclodiene insecticides Development of monoclonal immunoassay selective for chlorinated cyclodiene insecticides (en)
skos:prefLabel	Vývoj monoklonální imunonometody selektivní pro chlorované cyklodienové insekticidy (cs) Development of monoclonal immunoassay selective for chlorinated cyclodiene insecticides Development of monoclonal immunoassay selective for chlorinated cyclodiene insecticides (en)
skos:notation	RIV/60461373:22330/04:00012768!RIV/2005/MSM/223305/N
http://linked.open.../vavai/riv/strany	2776-2784
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM 223300006)
http://linked.open...iv/cisloPeriodika	10
http://linked.open...vai/riv/dodaniDat	2005
http://linked.open...aciTvurceVysledku	Holubová, Barbora
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta potravinářské a biochemické technologie
http://linked.open...dnocenehoVysledku	560245
http://linked.open...ai/riv/idVysledku	RIV/60461373:22330/04:00012768
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	chlorinated cyclodiene insecticides;ELISA;monoclonal antibodies (en)
http://linked.open.../riv/klicoveSlovo	chlorinated cyclodiene insecticides ELISA monoclonal antibodies
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[D00266D56CAE]
http://linked.open...i/riv/nazevZdroje	Journal of Agricultural and Food Chemistry
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...UplatneniVysledku	2004
http://linked.open...v/svazekPeriodika	52
http://linked.open...iv/tvurceVysledku	Abad, A. Mičková, Barbora Montoya, A. Lebedev, M. Manclus, J. Mercader, J. Miranda, M. Mojarrad, F. Primo, J.
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20223300006
issn	0021-8561
number of pages	9 (xsd:int)
http://localhost/t...ganizacniJednotka	22330

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 58 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software